Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3651546..3652087 Replicon chromosome
Accession NZ_CP043183
Organism Escherichia coli O16:H48 strain PG20180172

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag FYA19_RS18005 Protein ID WP_000615983.1
Coordinates 3651809..3652087 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag FYA19_RS18000 Protein ID WP_000729703.1
Coordinates 3651546..3651806 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA19_RS17980 3647221..3648006 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
FYA19_RS17985 3647978..3649690 + 1713 Protein_3504 flagellar biosynthesis protein FlhA -
FYA19_RS17990 3649914..3650411 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FYA19_RS17995 3650587..3651336 - 750 WP_000056849.1 C40 family peptidase -
FYA19_RS18000 3651546..3651806 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FYA19_RS18005 3651809..3652087 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FYA19_RS18010 3652243..3652983 + 741 WP_001225679.1 murein L,D-transpeptidase -
FYA19_RS18015 3652954..3653721 - 768 WP_000333380.1 class II glutamine amidotransferase -
FYA19_RS18020 3653927..3654505 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T137416 WP_000615983.1 NZ_CP043183:3651809-3652087 [Escherichia coli O16:H48]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T137416 NZ_CP056727:c2273392-2272907 [Enterobacter hormaechei]
GTGGGACGTGTAACCCCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTACTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT137416 WP_000729703.1 NZ_CP043183:3651546-3651806 [Escherichia coli O16:H48]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT137416 NZ_CP043183:3651546-3651806 [Escherichia coli O16:H48]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References