Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1793191..1793693 Replicon chromosome
Accession NZ_CP043183
Organism Escherichia coli O16:H48 strain PG20180172

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FYA19_RS08505 Protein ID WP_000767829.1
Coordinates 1793439..1793693 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FYA19_RS08500 Protein ID WP_001259255.1
Coordinates 1793191..1793442 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA19_RS08475 1788369..1789436 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FYA19_RS08480 1789436..1790506 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
FYA19_RS08485 1790503..1791807 - 1305 WP_000009594.1 histidinol dehydrogenase -
FYA19_RS08490 1791813..1792712 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FYA19_RS08495 1792858..1792908 - 51 WP_001364200.1 his operon leader peptide -
FYA19_RS08500 1793191..1793442 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FYA19_RS08505 1793439..1793693 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FYA19_RS08510 1793776..1794600 + 825 WP_000754737.1 SDR family oxidoreductase -
FYA19_RS08515 1794646..1795575 + 930 WP_000803351.1 LysR family transcriptional regulator -
FYA19_RS08520 1795790..1795852 + 63 WP_010723108.1 membrane protein YoeI -
FYA19_RS08525 1795842..1797200 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FYA19_RS08530 1797379..1798437 + 1059 WP_000492339.1 transport protein YeeE -
FYA19_RS08535 1798451..1798678 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T137400 WP_000767829.1 NZ_CP043183:1793439-1793693 [Escherichia coli O16:H48]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T137400 NZ_CP056717:c4402420-4402133 [Citrobacter sp. RHBSTW-00599]
ATGCCAATGGAGTTTGAGTGGGATGCAAACAAGGCACAAAGTAACCACCGTAAACATGGTATTCGCTTTGAGGACGCAAT
ATTGGTTTTCGACGATCCCCAACATCTGTCGCGGCAGGATCGGTATGAGAACGGTGAGTATCGCTGGCAGACCATTGGTC
TCGTTCATGGCATTGTGGTTATTCTGGTTGCCCATAGCGTTAGGTTTGAAAGTGGCTTTGAAGTCATTCGTATCATCAGT
GCCAGAAAGGCAGATCGTAAAGAGAGGAGTCGTTATGAACATAGTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT137400 WP_001259255.1 NZ_CP043183:1793191-1793442 [Escherichia coli O16:H48]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT137400 NZ_CP056717:c4402146-4401844 [Citrobacter sp. RHBSTW-00599]
ATGAACATAGTTAAACATAAACGCGGCAGCGTATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
TAAACCGGACGATGATATTGATTACAGCGACATTCCCGTGACCGAGGATGAGCTGTGGTCAGAAGCTCATCGCGGTAAGT
TTTTCCGTCCGTTGAAAACGCAGGCGTCGGTACGCATTGATGCCGATGTGATGGAATGGCTGAAGCGCCCCGGGAAGGGT
TACCAGACGCGTCTCAATGCCATTTTGCGCGAAGCGATGCTGCGCGAGCAAAACAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References