Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5003483..5004036 Replicon chromosome
Accession NZ_CP043181
Organism Escherichia coli O2:H6 strain PG20180057

Toxin (Protein)


Gene name higB Uniprot ID A0A0G9FUJ7
Locus tag FYA20_RS24440 Protein ID WP_000605680.1
Coordinates 5003767..5004036 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag FYA20_RS24435 Protein ID WP_000781370.1
Coordinates 5003483..5003767 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA20_RS24420 4998664..5001711 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
FYA20_RS24425 5001724..5002608 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
FYA20_RS24430 5002601..5003254 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
FYA20_RS24435 5003483..5003767 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
FYA20_RS24440 5003767..5004036 - 270 WP_000605680.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FYA20_RS24445 5004222..5005232 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
FYA20_RS24450 5005366..5007063 - 1698 WP_148886105.1 malate dehydrogenase -
FYA20_RS24455 5007220..5007357 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
FYA20_RS24460 5007459..5007674 - 216 WP_000495766.1 biofilm-dependent modulation protein -
FYA20_RS24465 5008019..5008450 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10181.73 Da        Isoelectric Point: 6.8582

>T137388 WP_000605680.1 NZ_CP043181:c5004036-5003767 [Escherichia coli O2:H6]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

>T137388 NZ_CP056716:c1059994-1059776 [Enterobacter asburiae]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACGCTTGAGCGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAGCTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TGACAATGAATAAACTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT137388 WP_000781370.1 NZ_CP043181:c5003767-5003483 [Escherichia coli O2:H6]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT137388 NZ_CP043181:c5003767-5003483 [Escherichia coli O2:H6]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FUJ7

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References