Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 4621995..4622220 Replicon chromosome
Accession NZ_CP043181
Organism Escherichia coli O2:H6 strain PG20180057

Toxin (Protein)


Gene name hokW Uniprot ID S1ELB5
Locus tag FYA20_RS22420 Protein ID WP_000813254.1
Coordinates 4622065..4622220 (+) Length 52 a.a.

Antitoxin (RNA)


Gene name sokW
Locus tag -
Coordinates 4621995..4622053 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA20_RS22395 4618077..4618508 + 432 WP_148886038.1 DUF977 family protein -
FYA20_RS22400 4618832..4619071 + 240 WP_148886040.1 hypothetical protein -
FYA20_RS22405 4619074..4619670 + 597 WP_148886041.1 hypothetical protein -
FYA20_RS22410 4619894..4621618 + 1725 WP_148886043.1 DUF2326 domain-containing protein -
- 4621995..4622053 - 59 - - Antitoxin
FYA20_RS22420 4622065..4622220 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD Toxin
FYA20_RS22425 4622387..4622659 + 273 WP_001341173.1 hypothetical protein -
FYA20_RS22430 4622661..4623710 + 1050 WP_148886047.1 DUF968 domain-containing protein -
FYA20_RS22435 4623723..4624097 + 375 WP_148886048.1 RusA family crossover junction endodeoxyribonuclease -
FYA20_RS22440 4624094..4624915 + 822 WP_034173000.1 antitermination protein -
FYA20_RS22445 4625142..4625339 + 198 WP_000917768.1 hypothetical protein -
FYA20_RS22450 4625489..4626547 + 1059 WP_148886050.1 site-specific DNA-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4610051..4622659 12608


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(6-47)


Sequences

Toxin        


Download         Length: 52 a.a.        Molecular weight: 5736.89 Da        Isoelectric Point: 6.1531

>T137385 WP_000813254.1 NZ_CP043181:4622065-4622220 [Escherichia coli O2:H6]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Download         Length: 156 bp

>T137385 NZ_CP043181:4622065-4622220 [Escherichia coli O2:H6]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 59 bp

>AT137385 NZ_CP043181:c4622053-4621995 [Escherichia coli O2:H6]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TMW0

Antitoxin

Download structure file

References