Detailed information of TA system TA137382

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3679359..3680053
• Replicon: chromosome
• Accession: NZ_CP043181
• Organism: Escherichia coli O2:H6 strain PG20180057

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: B7MQ69
• Locus tag: FYA20_RS17760
• Protein ID: WP_001263501.1
• Coordinates: 3679655..3680053 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: FYA20_RS17755
• Protein ID: WP_000554755.1
• Coordinates: 3679359..3679652 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA20_RS17730	3674698..3675195	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
FYA20_RS17735	3675190..3675684	-	495	WP_139174989.1	hypothetical protein	-
FYA20_RS17740	3675712..3677424	-	1713	Protein_3399	flagellar biosynthesis protein FlhA	-
FYA20_RS17745	3677396..3678181	+	786	WP_187424783.1	putative lateral flagellar export/assembly protein LafU	-
FYA20_RS17750	3678252..3679307	+	1056	WP_001226170.1	DNA polymerase IV	-
FYA20_RS17755	3679359..3679652	+	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FYA20_RS17760	3679655..3680053	+	399	WP_001263501.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FYA20_RS17765	3680063..3680515	+	453	WP_001059897.1	GNAT family N-acetyltransferase	-
FYA20_RS17770	3680705..3681844	+	1140	WP_000521578.1	RNA ligase RtcB family protein	-
FYA20_RS17775	3681841..3682455	+	615	WP_000602123.1	peptide chain release factor H	-
FYA20_RS17780	3682512..3683969	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
FYA20_RS17785	3684230..3684688	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3657432..3680515	23083

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0950

>T137382 WP_001263501.1 NZ_CP043181:3679655-3680053 [Escherichia coli O2:H6]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T137382 NZ_CP056711:c3990796-3990386 [Enterobacter asburiae]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTTGCGGC
CTTTGTTTTACTGATGCCGTGGCCGCTGAGCTATACGCCGCTGTGGCTGCTGTTACTGTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATTAAATTACTGATGGATTCCCGCCTGCGCTGGCAGGGTAAA
GAGTGGGATATCCTCGGTATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGTTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTGTGGCTGGCGGCTGACAGTATGGATGCTGCCGAGTGGCGAGACCTGCGCCGGATGCTGTTGCAACAAACGA
CGCAGGGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT137382 WP_000554755.1 NZ_CP043181:3679359-3679652 [Escherichia coli O2:H6]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT137382 NZ_CP056711:c3991043-3990777 [Enterobacter asburiae]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTTGAATATGAGTATGACAGCTTAAGCGATGATGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A376DBU4

Antitoxin

• AlphaFold DB: A0A829G9H5