Detailed information of TA system TA137375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2727890..2728492
• Replicon: chromosome
• Accession: NZ_CP043181
• Organism: Escherichia coli O2:H6 strain PG20180057

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: FYA20_RS13170
• Protein ID: WP_000897302.1
• Coordinates: 2727890..2728201 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FYA20_RS13175
• Protein ID: WP_000356397.1
• Coordinates: 2728202..2728492 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA20_RS13145	2723804..2724403	+	600	WP_001314324.1	glucose-1-phosphatase	-
FYA20_RS13150	2724397..2725269	+	873	WP_148885932.1	virulence factor BrkB family protein	-
FYA20_RS13155	2725266..2725703	+	438	WP_000560990.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FYA20_RS13160	2725748..2726689	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FYA20_RS13165	2726753..2727661	-	909	WP_001331553.1	alpha/beta hydrolase	-
FYA20_RS13170	2727890..2728201	+	312	WP_000897302.1	hypothetical protein	Toxin
FYA20_RS13175	2728202..2728492	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FYA20_RS13185	2728851..2729129	+	279	WP_001296612.1	hypothetical protein	-
FYA20_RS13195	2729525..2729743	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
FYA20_RS13200	2729959..2730888	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
FYA20_RS13205	2730885..2731520	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FYA20_RS13210	2731517..2732419	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T137375 WP_000897302.1 NZ_CP043181:2727890-2728201 [Escherichia coli O2:H6]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T137375 NZ_CP056711:c84092-83772 [Enterobacter asburiae]
ATGCATATTTCAACTGTACCGGCAACGGTGACGGTTTCGTCACGCCTGTCTCCTGTCCAAGTTTGGCAAAAACTATTAAC
GTATATTTTAGAGCATCATTACGGCCTCACGATCAACGACACACCGTTCAGTAACGATACGACGATTCAGGAACATATAG
AGGCTGGAGTGAATCTTACTGATGCTGTTAATTTCCTTGTGGAACGATTCGAGCTTGTGCGCATTGACCAGAAGGGTTTC
TCCTGGCAGGATCAAGAACCTTGGATAACATCTTTGGATGTTCATCGGGCACAGTTCAACCTTGGCTTGAAACGCTCATA
A

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT137375 WP_000356397.1 NZ_CP043181:2728202-2728492 [Escherichia coli O2:H6]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT137375 NZ_CP043181:2728202-2728492 [Escherichia coli O2:H6]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416