Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1914239..1915038 Replicon chromosome
Accession NZ_CP043181
Organism Escherichia coli O2:H6 strain PG20180057

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag FYA20_RS09180 Protein ID WP_000347251.1
Coordinates 1914574..1915038 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag FYA20_RS09175 Protein ID WP_001296435.1
Coordinates 1914239..1914574 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA20_RS09160 1910024..1910794 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
FYA20_RS09165 1910810..1912144 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
FYA20_RS09170 1912519..1914090 + 1572 WP_001273939.1 galactarate dehydratase -
FYA20_RS09175 1914239..1914574 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
FYA20_RS09180 1914574..1915038 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
FYA20_RS09185 1915093..1915902 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
FYA20_RS09190 1916151..1917431 + 1281 WP_000681930.1 tagatose-bisphosphate aldolase subunit KbaZ -
FYA20_RS09195 1917454..1917927 + 474 WP_001298322.1 PTS N-acetylgalactosamine transporter subunit IIB -
FYA20_RS09200 1917938..1918717 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
FYA20_RS09205 1918707..1919585 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
FYA20_RS09210 1919603..1920037 + 435 WP_000948834.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T137374 WP_000347251.1 NZ_CP043181:1914574-1915038 [Escherichia coli O2:H6]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T137374 NZ_CP043181:1914574-1915038 [Escherichia coli O2:H6]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT137374 WP_001296435.1 NZ_CP043181:1914239-1914574 [Escherichia coli O2:H6]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT137374 NZ_CP056708:4918-5184 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References