Detailed information of TA system TA137024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 74394..74997
• Replicon: plasmid pAP43-2
• Accession: NZ_CP043054
• Organism: Acinetobacter pittii strain AP43

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FXO17_RS20710
• Protein ID: WP_000288004.1
• Coordinates: 74668..74997 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FXO17_RS20705
• Protein ID: WP_001258993.1
• Coordinates: 74394..74681 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FXO17_RS20660	69954..70256	+	303	WP_079283729.1	hypothetical protein	-
FXO17_RS20665	70317..70778	+	462	WP_000378528.1	hypothetical protein	-
FXO17_RS20670	70747..71388	+	642	WP_000372120.1	hypothetical protein	-
FXO17_RS20675	71479..72002	+	524	Protein_67	DUF2280 domain-containing protein	-
FXO17_RS20680	71986..72495	+	510	Protein_68	Terminase-like domain protein	-
FXO17_RS20930	72850..73173	+	324	WP_005804845.1	hypothetical protein	-
FXO17_RS20690	73245..73421	+	177	WP_002120129.1	hypothetical protein	-
FXO17_RS20695	73430..73753	+	324	Protein_71	DUF4236 domain-containing protein	-
FXO17_RS20705	74394..74681	-	288	WP_001258993.1	helix-turn-helix domain-containing protein	Antitoxin
FXO17_RS20710	74668..74997	-	330	WP_000288004.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FXO17_RS20715	75052..75321	-	270	WP_000453245.1	transcriptional regulator	-
FXO17_RS20720	75528..76130	+	603	WP_000096286.1	hypothetical protein	-
FXO17_RS20725	76187..76405	+	219	WP_000994925.1	hypothetical protein	-
FXO17_RS20730	76498..76695	+	198	WP_025464206.1	hypothetical protein	-
FXO17_RS20735	76826..77758	-	933	WP_000743263.1	IS5 family transposase	-
FXO17_RS20740	77925..78110	+	186	WP_000755724.1	hypothetical protein	-
FXO17_RS20745	78113..78625	+	513	WP_000464612.1	Fic family protein	-
FXO17_RS20750	78867..79195	-	329	Protein_81	DUF4236 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92276	92276
	-	flank	IS/Tn	-	-	76826..77758	932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-90)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12571.69 Da
• Isoelectric Point: 8.8102

>T137024 WP_000288004.1 NZ_CP043054:c74997-74668 [Acinetobacter pittii]
MYTIEYYSEDVQDEILTLPDGLLARYFNLTDRMVIYGANLGEPHTLPMSKGLFELRLKSQEGIARVMYCTLVGKRIVMLH
SFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 330 bp

>T137024 NZ_CP043054:c74997-74668 [Acinetobacter pittii]
ATGTATACAATTGAATATTACAGCGAAGACGTTCAGGATGAGATTCTTACATTACCTGATGGTCTTTTGGCTCGATATTT
TAACTTAACTGACCGTATGGTGATTTACGGCGCAAACTTAGGAGAACCTCACACATTACCAATGAGTAAAGGACTATTTG
AGTTGCGCTTAAAATCTCAAGAAGGTATTGCTCGTGTTATGTATTGCACACTAGTCGGTAAACGCATTGTAATGCTCCAT
AGTTTTGTTAAGAAAACCCAAAAAACACCTAAGCAAGATCTAAACCTAGCACTTGACCGCATGAAAGAGGTGAAAAATGC
GAACACATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10565.30 Da
• Isoelectric Point: 10.1453

>AT137024 WP_001258993.1 NZ_CP043054:c74681-74394 [Acinetobacter pittii]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTARRDAGLSQAQIAERMGTSVPAVSRLEKALITGKPSPSIATLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT137024 NZ_CP043054:c74681-74394 [Acinetobacter pittii]
ATGCGAACACATGAAGAAATGAAAGCACTTGCATTATCTCGTTCAGCAGTGCGTACCGAATACGAACGAATTGAACGTGA
AGAAATGCCCCTGCTCGATATGGTACTTACTGCACGTCGAGATGCAGGTCTATCCCAAGCGCAAATCGCGGAACGCATGG
GAACAAGTGTACCTGCCGTTTCACGGCTAGAAAAAGCATTGATCACAGGCAAACCATCACCATCTATTGCCACATTGCAA
AAATATGCTGCGGCAATTGGTAAACATGTTGAAGTGCGTTTTGTCTAA