Detailed information of TA system TA137023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 33946..34597
• Replicon: plasmid pAP43-2
• Accession: NZ_CP043054
• Organism: Acinetobacter pittii strain AP43

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FXO17_RS20515
• Protein ID: WP_000269908.1
• Coordinates: 34241..34597 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A009MPA9
• Locus tag: FXO17_RS20510
• Protein ID: WP_032001945.1
• Coordinates: 33946..34248 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FXO17_RS20475	29100..29999	-	900	WP_001180108.1	LysR family transcriptional regulator	-
FXO17_RS20480	30135..30875	+	741	WP_001059480.1	SDR family oxidoreductase	-
FXO17_RS20485	30949..31947	+	999	WP_002120211.1	zinc-binding alcohol dehydrogenase family protein	-
FXO17_RS20490	31968..32252	+	285	WP_000155817.1	antibiotic biosynthesis monooxygenase	-
FXO17_RS20495	32387..32608	+	222	Protein_33	IS3 family transposase	-
FXO17_RS20500	32953..33180	-	228	WP_000555567.1	hypothetical protein	-
FXO17_RS20510	33946..34248	-	303	WP_032001945.1	XRE family transcriptional regulator	Antitoxin
FXO17_RS20515	34241..34597	-	357	WP_000269908.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FXO17_RS20520	35155..35838	+	684	WP_000478902.1	hypothetical protein	-
FXO17_RS20525	36043..36571	+	529	Protein_38	IS3 family transposase	-
FXO17_RS20530	36814..37134	+	321	WP_001081101.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FXO17_RS20535	37405..37863	-	459	Protein_40	AAA family ATPase	-
FXO17_RS20540	37872..38897	-	1026	WP_010326927.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92276	92276

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13622.56 Da
• Isoelectric Point: 6.4651

>T137023 WP_000269908.1 NZ_CP043054:c34597-34241 [Acinetobacter pittii]
MWTVITTDLFNEWLEQQDESTQEKVLTALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPFRQAI
VLCIGDKGGKKRFYKEMLNIAEQQYELHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T137023 NZ_CP043054:c34597-34241 [Acinetobacter pittii]
ATGTGGACAGTCATCACGACAGATCTGTTTAATGAATGGCTTGAACAGCAAGACGAATCAACACAAGAGAAAGTCTTAAC
CGCTTTAGTTGTTTTACAGCAGCAGGGACCAAGTTTAGGTCGCCCTTTAGTTGATACCGTATATGACTCTAAATTTACCA
ATATGAAAGAGCTACGCGTTCAGCATCGAGGAAAACCCCTGAGAGCCTTTTTCGCATTTGATCCATTTCGACAGGCAATT
GTGCTGTGCATCGGTGATAAAGGCGGTAAAAAGCGTTTCTATAAAGAGATGCTGAACATTGCAGAGCAGCAATACGAACT
TCATCTCTCAACCCTTGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11219.99 Da
• Isoelectric Point: 6.4732

>AT137023 WP_032001945.1 NZ_CP043054:c34248-33946 [Acinetobacter pittii]
MAKTLQELLASRSPESQTRIQQMADELLLETQLHFIREELEISQKELAATLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT137023 NZ_CP043054:c34248-33946 [Acinetobacter pittii]
ATGGCTAAAACCTTACAGGAATTATTGGCTAGTCGCTCTCCAGAGAGCCAAACTCGTATTCAACAAATGGCTGACGAGCT
ACTTTTAGAAACTCAGCTTCACTTTATTCGAGAAGAATTAGAGATTTCCCAAAAGGAACTGGCTGCAACTCTTGGAATCA
AACAACCTTCATTGTCAGCAATCGAAAACCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAAGCTATG
GGGGGGAAACTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAATGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A009MPA9