Detailed information of TA system TA137022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 186591..187176
• Replicon: plasmid pAP43-OXA58-NDM1
• Accession: NZ_CP043053
• Organism: Acinetobacter pittii strain AP43

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: FXO17_RS20005
• Protein ID: WP_000897307.1
• Coordinates: 186591..186911 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FXO17_RS20010
• Protein ID: WP_000369781.1
• Coordinates: 186904..187176 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FXO17_RS19960	181943..182476	+	534	Protein_195	IS6-like element IS1006 family transposase	-
FXO17_RS19965	182604..182942	-	339	WP_032073153.1	hypothetical protein	-
FXO17_RS19970	183001..183771	+	771	WP_032073134.1	hypothetical protein	-
FXO17_RS19975	184012..184299	+	288	WP_000438825.1	BrnT family toxin	-
FXO17_RS19980	184286..184600	+	315	WP_004728120.1	BrnA antitoxin family protein	-
FXO17_RS19985	184689..185219	-	531	WP_000172759.1	hypothetical protein	-
FXO17_RS19990	185368..185670	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FXO17_RS19995	185671..185952	+	282	WP_000985609.1	putative addiction module antidote protein	-
FXO17_RS20000	186032..186451	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
FXO17_RS20005	186591..186911	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FXO17_RS20010	186904..187176	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
FXO17_RS20015	187669..189144	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
FXO17_RS20020	189200..190084	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
FXO17_RS20025	190266..190970	-	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
FXO17_RS20030	190903..191778	-	876	WP_114190151.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaNDM-1 / aph(3')-VIa / aac(3)-IId / blaOXA-58 / msr(E) / mph(E) / floR	-	1..268263	268263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T137022 WP_000897307.1 NZ_CP043053:186591-186911 [Acinetobacter pittii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T137022 NZ_CP056410:74553-75083 [Citrobacter sp. RHBSTW-00535]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGA
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT137022 WP_000369781.1 NZ_CP043053:186904-187176 [Acinetobacter pittii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT137022 NZ_CP056410:74280-74549 [Citrobacter sp. RHBSTW-00535]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3