Detailed information of TA system TA136948

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4389408..4390072
• Replicon: chromosome
• Accession: NZ_CP043009
• Organism: Citrobacter portucalensis strain WCHCP085039

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FXN82_RS21700
• Protein ID: WP_032942116.1
• Coordinates: 4389770..4390072 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5A9BY49
• Locus tag: FXN82_RS21695
• Protein ID: WP_008786950.1
• Coordinates: 4389408..4389749 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FXN82_RS21665	4384814..4385572	+	759	WP_003844769.1	phosphonate C-P lyase system protein PhnK	-
FXN82_RS21670	4385791..4386477	+	687	WP_063941249.1	phosphonate C-P lyase system protein PhnL	-
FXN82_RS21675	4386474..4387610	+	1137	WP_126319496.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
FXN82_RS21680	4387613..4388167	+	555	WP_148362556.1	ribose 1,5-bisphosphokinase	-
FXN82_RS21685	4388154..4388588	+	435	WP_126319498.1	aminoalkylphosphonate N-acetyltransferase	-
FXN82_RS21690	4388597..4389355	+	759	WP_148362557.1	phosphonate metabolism protein PhnP	-
FXN82_RS21695	4389408..4389749	-	342	WP_008786950.1	HigA family addiction module antidote protein	Antitoxin
FXN82_RS21700	4389770..4390072	-	303	WP_032942116.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FXN82_RS21705	4390228..4390557	-	330	WP_003031807.1	hypothetical protein	-
FXN82_RS21710	4390627..4392891	-	2265	WP_148362558.1	hybrid sensor histidine kinase/response regulator	-
FXN82_RS21720	4393006..4394529	+	1524	WP_003844755.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-98)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11860.49 Da
• Isoelectric Point: 9.9581

>T136948 WP_032942116.1 NZ_CP043009:c4390072-4389770 [Citrobacter portucalensis]
MSKSLNIRNFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWINGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T136948 NZ_CP056365:c4685810-4685451 [Citrobacter freundii]
ATGACGAAACCACTTTACTGGGTTGGGCAGGCCAGAAAAGACCTGATTGCAATGCCAGAACACGTTCGGGATACGTTTGG
GTTTGCTCTTTGGCTTGCGCAACAGGGTAAACAGCATTCACAGACTAAACCATTAAAAGGATTTGGTGGAGCGGGGGTTC
TGGAAGTCGTTGAGGATTATCACGGCAACGCCTGGCGGGCGGTTTACACCATCCAGTTGAAAAATGCGGTTTATGTTCTC
CATGTTTTTCAGAAGAAGTCCGTTTCCGGGAAAGCAACCCCGAAACCTGAAATTGACCTGATTTATCAGCGGCTCAAGGC
TGCACAGCGCCATGCGCAGGAGAGTGGATATGTCACATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12986.83 Da
• Isoelectric Point: 6.4743

>AT136948 WP_008786950.1 NZ_CP043009:c4389749-4389408 [Citrobacter portucalensis]
MKQASRKPTTVGDILQYEYLEPLDLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRDVGAKKVA

Nucleotide

• Length: 342 bp

>AT136948 NZ_CP056365:c4685461-4685144 [Citrobacter freundii]
ATGTCACATGATGTCGAAACCAGTAGCGGTAATGTTTACGCCGATTTAGGTGCGTATGACGCAGAACAGATGCAGTTGAA
AGCGCAGCTGGCAATGGTGATTGGTGAGATTTTGCAACGCAAAGGGTTAACGCAGCAGCAGGCCGCGCAGTTACTGGGAA
TGACACAGCCTAAACTTTCCAATCTGCTACGCGGCCAGTTTCGCGGTATCAGTGAAGCAAAGATGCTCGAATGCCTGGTG
AGGCTGGGCCGTGATGTAGAGATTGTTGTCGGGAAAGAGGGCAATATGTCAGGAAAGCTGAAAGTGAAATTTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A5A9BY49