Detailed information of TA system TA136915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1473765..1474411
• Replicon: chromosome
• Accession: NZ_CP043004
• Organism: Aggregatibacter actinomycetemcomitans strain HK_973

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FXN59_RS07700
• Protein ID: WP_151294617.1
• Coordinates: 1473765..1474115 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: X2JT85
• Locus tag: FXN59_RS07705
• Protein ID: WP_005540827.1
• Coordinates: 1474112..1474411 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FXN59_RS07680 (FXN59_07680)	1468947..1469192	-	246	WP_005540815.1	YgjV family protein	-
FXN59_RS07685 (FXN59_07685)	1469385..1470722	-	1338	WP_151294613.1	argininosuccinate synthase	-
FXN59_RS07690 (FXN59_07690)	1470810..1471637	-	828	WP_148326563.1	SDR family NAD(P)-dependent oxidoreductase	-
FXN59_RS07695 (FXN59_07695)	1471994..1473577	+	1584	WP_151294615.1	peptide chain release factor 3	-
FXN59_RS07700 (FXN59_07700)	1473765..1474115	+	351	WP_151294617.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FXN59_RS07705 (FXN59_07705)	1474112..1474411	+	300	WP_005540827.1	helix-turn-helix transcriptional regulator	Antitoxin
FXN59_RS07710 (FXN59_07710)	1474447..1477380	-	2934	WP_148326565.1	bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase	-
FXN59_RS12580	1477580..1477759	+	180	WP_233443981.1	CRISPR-associated protein Cas3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13506.68 Da
• Isoelectric Point: 9.6304

>T136915 WP_151294617.1 NZ_CP043004:1473765-1474115 [Aggregatibacter actinomycetemcomitans]
MWAVILTERFETWLNQQPIKTQECVLANLVRLEHFGPNLACPYVDSVAGSIHSNMKELRIQHSGRAIRLFFAFDPKRRAI
VLCGGDKGNDKRFYRTMIRIADQELNHYLATMEQKK

Nucleotide

• Length: 351 bp

>T136915 NZ_CP043004:1473765-1474115 [Aggregatibacter actinomycetemcomitans]
ATGTGGGCGGTGATTTTAACGGAACGATTTGAAACTTGGTTAAATCAACAGCCGATAAAAACGCAGGAATGTGTTTTGGC
AAACTTGGTACGGTTGGAACATTTTGGTCCGAATTTAGCTTGTCCTTATGTAGATAGCGTAGCAGGGTCAATTCATAGCA
ACATGAAAGAACTACGCATTCAGCATTCGGGAAGAGCAATTCGACTCTTTTTTGCTTTTGATCCAAAGCGTAGAGCCATT
GTTTTATGTGGTGGAGATAAAGGCAATGACAAGCGGTTTTACCGTACGATGATTAGAATTGCAGATCAAGAATTGAATCA
TTATTTGGCAACCATGGAGCAAAAAAAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11342.12 Da
• Isoelectric Point: 5.8598

>AT136915 WP_005540827.1 NZ_CP043004:1474112-1474411 [Aggregatibacter actinomycetemcomitans]
MKTLQQVIDERSVESRQRIQTMAEDLILETGLQLLREELNISQQQLATILGVTQPAINQLEQRGNEIKLATLKRYIEAMG
GKLRLSVEMPDGHQRVFQI

Nucleotide

• Length: 300 bp

>AT136915 NZ_CP056308:32483-32713 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: X2JT85