Detailed information of TA system TA136845

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2034385..2034610
• Replicon: chromosome
• Accession: NZ_CP042980
• Organism: Shigella flexneri Y strain PE577

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: FW259_RS10770
• Protein ID: WP_000935258.1
• Coordinates: 2034385..2034597 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2034552..2034610 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FW259_RS10705	2029697..2030047	-	351	WP_005049241.1	IS66 family insertion sequence element accessory protein TnpB	-
FW259_RS10710	2030044..2030718	-	675	WP_004967157.1	IS66 family insertion sequence hypothetical protein	-
FW259_RS10715	2030817..2031032	-	216	WP_000839572.1	class II holin family protein	-
FW259_RS10745	2031833..2032516	-	684	WP_005049343.1	antiterminator	-
FW259_RS10750	2032513..2032878	-	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
FW259_RS10755	2032879..2033937	-	1059	WP_001265248.1	DUF968 domain-containing protein	-
FW259_RS10760	2033939..2034217	-	279	WP_011069426.1	hypothetical protein	-
FW259_RS10770	2034385..2034597	-	213	WP_000935258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2034552..2034610	+	59	-	-	Antitoxin
FW259_RS10785	2035192..2035608	-	417	WP_005069274.1	hypothetical protein	-
FW259_RS10790	2035635..2035775	+	141	Protein_2052	DUF4224 domain-containing protein	-
FW259_RS10795	2035775..2036812	+	1038	WP_004974968.1	tyrosine-type recombinase/integrase	-
FW259_RS10805	2037023..2037820	+	798	WP_001325918.1	DgsA anti-repressor MtfA	-
FW259_RS10815	2038158..2039420	+	1263	Protein_2055	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	2002535..2071698	69163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7856.42 Da
• Isoelectric Point: 8.7469

>T136845 WP_000935258.1 NZ_CP042980:c2034597-2034385 [Shigella flexneri Y]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T136845 NZ_CP042980:c2034597-2034385 [Shigella flexneri Y]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT136845 NZ_CP042980:2034552-2034610 [Shigella flexneri Y]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin