Detailed information of TA system TA136806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4755460..4756062
• Replicon: chromosome
• Accession: NZ_CP042973
• Organism: Escherichia coli strain CFSAN061768

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CA694_RS22630
• Protein ID: WP_000897305.1
• Coordinates: 4755751..4756062 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CA694_RS22625
• Protein ID: WP_000356395.1
• Coordinates: 4755460..4755750 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA694_RS22590 (4751084)	4751084..4751986	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CA694_RS22595 (4751983)	4751983..4752618	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CA694_RS22600 (4752615)	4752615..4753544	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
CA694_RS22605 (4753726)	4753726..4753968	-	243	WP_001309881.1	CopG family transcriptional regulator	-
CA694_RS22610 (4754187)	4754187..4754405	-	219	WP_001295676.1	CopG family transcriptional regulator	-
CA694_RS22615 (4754824)	4754824..4755102	-	279	WP_001315112.1	hypothetical protein	-
CA694_RS22620 (4755154)	4755154..4755375	-	222	WP_001550354.1	hypothetical protein	-
CA694_RS22625 (4755460)	4755460..4755750	-	291	WP_000356395.1	NadS family protein	Antitoxin
CA694_RS22630 (4755751)	4755751..4756062	-	312	WP_000897305.1	hypothetical protein	Toxin
CA694_RS22635 (4756291)	4756291..4757199	+	909	WP_001550353.1	alpha/beta hydrolase	-
CA694_RS24400 (4757367)	4757367..4758281	-	915	WP_109553727.1	transposase	-
CA694_RS24405 (4758294)	4758294..4759181	-	888	Protein_4440	hypothetical protein	-
CA694_RS22645 (4759597)	4759597..4760538	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CA694_RS22650 (4760583)	4760583..4761020	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T136806 WP_000897305.1 NZ_CP042973:c4756062-4755751 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T136806 NZ_CP042973:c4756062-4755751 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT136806 WP_000356395.1 NZ_CP042973:c4755750-4755460 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT136806 NZ_CP042973:c4755750-4755460 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7