Detailed information of TA system TA136785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1113807..1114534
• Replicon: chromosome
• Accession: NZ_CP042973
• Organism: Escherichia coli strain CFSAN061768

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: CA694_RS05280
• Protein ID: WP_000547555.1
• Coordinates: 1113807..1114118 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CA694_RS05285
• Protein ID: WP_000126294.1
• Coordinates: 1114115..1114534 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA694_RS05255 (1109742)	1109742..1111451	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
CA694_RS05260 (1111461)	1111461..1112003	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
CA694_RS05265 (1112003)	1112003..1112770	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
CA694_RS05270 (1112767)	1112767..1113177	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
CA694_RS05275 (1113170)	1113170..1113640	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
CA694_RS05280 (1113807)	1113807..1114118	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
CA694_RS05285 (1114115)	1114115..1114534	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
CA694_RS05290 (1114613)	1114613..1116037	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
CA694_RS05295 (1116046)	1116046..1117503	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
CA694_RS05300 (1117763)	1117763..1118773	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
CA694_RS05305 (1118922)	1118922..1119449	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T136785 WP_000547555.1 NZ_CP042973:1113807-1114118 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T136785 NZ_CP042973:1113807-1114118 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT136785 WP_000126294.1 NZ_CP042973:1114115-1114534 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT136785 NZ_CP056232:4705897-4706148 [Citrobacter freundii]
ATGCCGCATATCATTTTAAGCGATACCACCGCCAGTGTGAGTGAGTTGAAAAAGAACCCGATGGCGACGGTTAGCGCAGG
GGATGGGTTTCCGGTCGCAATCCTGAACCGTAACCAGCCCGCCTTTTATTGTGTGCCAGCAGAGCTTTACGAAAAAATGC
TCGATGCGCTGGATGACCAACAGCTGGTTAAATTAGTCCATGAACGTAGCCAACAACCCCTGGTGGATGTGGATCTGGAC
AGCTACTTATGA

Structures

Toxin

• AlphaFold DB: V0YLE2