Detailed information of TA system TA136778

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 65793..66032
• Replicon: plasmid pCFSAN061769_03
• Accession: NZ_CP042972
• Organism: Escherichia coli strain CFSAN061769

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: CA697_RS24800
• Protein ID: WP_023144756.1
• Coordinates: 65898..66032 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	65793..65853 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA697_RS24780 (63323)	63323..64069	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
CA697_RS24785 (64124)	64124..64684	+	561	WP_000139341.1	fertility inhibition protein FinO	-
CA697_RS24790 (64815)	64815..65027	+	213	WP_013023861.1	hypothetical protein	-
CA697_RS24795 (65540)	65540..65826	+	287	Protein_78	DUF2726 domain-containing protein	-
- (65793)	65793..65853	-	61	NuclAT_2	-	Antitoxin
- (65793)	65793..65853	-	61	NuclAT_2	-	Antitoxin
- (65793)	65793..65853	-	61	NuclAT_2	-	Antitoxin
- (65793)	65793..65853	-	61	NuclAT_2	-	Antitoxin
CA697_RS24800 (65898)	65898..66032	+	135	WP_023144756.1	Hok/Gef family protein	Toxin
CA697_RS24805 (66329)	66329..66583	+	255	WP_000083850.1	replication regulatory protein RepA	-
CA697_RS24810 (66689)	66689..66820	+	132	Protein_81	protein CopA/IncA	-
CA697_RS24815 (66820)	66820..66894	+	75	WP_031943482.1	RepA leader peptide Tap	-
CA697_RS24820 (66887)	66887..67585	+	699	WP_143366464.1	plasmid replication initiator RepA	-
CA697_RS24825 (68287)	68287..68427	+	141	WP_001333237.1	hypothetical protein	-
CA697_RS24830 (68525)	68525..69178	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
CA697_RS24835 (69271)	69271..69528	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
CA697_RS24840 (69461)	69461..69862	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
CA697_RS24845 (70147)	70147..70404	+	258	WP_021521885.1	DUF262 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / sitABCD	iucA / iucB / iucC / iucD / iutA	1..129967	129967

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T136778 WP_023144756.1 NZ_CP042972:65898-66032 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T136778 NZ_CP056229:4446303-4446581 [Citrobacter freundii]
ATGAATAAACTTACTCGGTACGATCCCGCAACTGCGCTGGTTGATGATGAAGAAATTGCCGTATTTATGGCGGATGCCCT
CGAAACAGGCGATGCAGCGTACATTGCAAAAGCCCTGGGCGTGGTGGCTCGCGCTAAGGGAATGGCGAACATCGCGGCAG
AAACGGGATTATCCCGCGAACAACTTTATCGCTCTTTTAGTGAGAAAGGTAACCCAACGCTGAAAACTACGCTTGCAGTG
ATGAAGGCGCTCGGTATTGAATTGACACTTAAAAAATAG

Antitoxin

Nucleotide

• Length: 61 bp

>AT136778 NZ_CP042972:c65853-65793 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin