Detailed information of TA system TA136771

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4785842..4786444
• Replicon: chromosome
• Accession: NZ_CP042969
• Organism: Escherichia coli strain CFSAN061769

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CA697_RS23035
• Protein ID: WP_000897305.1
• Coordinates: 4786133..4786444 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CA697_RS23030
• Protein ID: WP_000356397.1
• Coordinates: 4785842..4786132 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA697_RS23005 (4781768)	4781768..4782670	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CA697_RS23010 (4782667)	4782667..4783302	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CA697_RS23015 (4783299)	4783299..4784228	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CA697_RS23020 (4784558)	4784558..4784800	-	243	WP_001087409.1	protein YiiF	-
CA697_RS23025 (4785019)	4785019..4785237	-	219	WP_001295676.1	CopG family transcriptional regulator	-
CA697_RS23030 (4785842)	4785842..4786132	-	291	WP_000356397.1	NadS family protein	Antitoxin
CA697_RS23035 (4786133)	4786133..4786444	-	312	WP_000897305.1	hypothetical protein	Toxin
CA697_RS23040 (4786673)	4786673..4787581	+	909	WP_001162704.1	alpha/beta hydrolase	-
CA697_RS23045 (4787645)	4787645..4788586	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CA697_RS23050 (4788631)	4788631..4789068	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
CA697_RS23055 (4789065)	4789065..4789937	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CA697_RS23060 (4789931)	4789931..4790530	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4784558..4792896	8338
	-	flank	IS/Tn	-	-	4790637..4791845	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T136771 WP_000897305.1 NZ_CP042969:c4786444-4786133 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T136771 NZ_CP056229:c3458942-3458622 [Citrobacter freundii]
ATGCAAATCTCAACTGTATCGGCCACGGTGTCGGTTTCGTCACATCTGTCACCCGTACAGGTCTGGCGGCAACTGTTAAC
GTATCTGTTAAAACACCATTACGGCCTGACGCTTAATGACACGCCATTCCATAATGACACGGCCATTCAGGAGCATATCG
AAGCGGAAATAACACTGTCTGATGCGGTGAATTTCCTGGTAGAACGTTATGAGTTGGTGCGTATTGACCGCAAAGGATTC
ACTTGGCAGGAGCAGACGCCATTCCTTACCGCGACCGATATTCTCAGAGCAAGGCGAGCAACCGGACTAATGAATACTTA
A

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT136771 WP_000356397.1 NZ_CP042969:c4786132-4785842 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT136771 NZ_CP056229:c3459345-3459010 [Citrobacter freundii]
ATGAATAACGACGCAATCCAAACTTGGGGTCTTAGACGAGATATCACTCCGTGTTTCGGATCCCGACTGGTGCAGGAAGG
TCATCGCCTGCATTTTCTGGCTGACCGGGCAGGATTCACCGGCTCATTCAGTGAGGTGCAGACCCTACAACTGGATGAAG
CCTTCCCGCATTTTGTCGCACATCTGGAGCTGATGCTGCTCTCCTGTGAGCTGAATCCCCGGTACGCGCACTGCGTCACG
CTGTACCGTAACGGGTTGACCTGTGAAGCCGATACGCTGGGCAGTTATGGGTATGTGTATATCGCAATTTATCCCACAAA
TCAGGCTAAAGACTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7