Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3835792..3836333 Replicon chromosome
Accession NZ_CP042969
Organism Escherichia coli strain CFSAN061769

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag CA697_RS18530 Protein ID WP_000615976.1
Coordinates 3836055..3836333 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CA697_RS18525 Protein ID WP_000729704.1
Coordinates 3835792..3836052 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA697_RS18500 (3830828) 3830828..3831967 + 1140 WP_000785664.1 flagellar type III secretion system protein FlhB -
CA697_RS18505 (3831951) 3831951..3834044 + 2094 WP_001140827.1 flagellar biosynthesis protein FlhA -
CA697_RS18510 (3834149) 3834149..3834427 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
CA697_RS18515 (3834420) 3834420..3834776 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
CA697_RS18520 (3834833) 3834833..3835606 - 774 WP_000543899.1 C40 family peptidase -
CA697_RS18525 (3835792) 3835792..3836052 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CA697_RS18530 (3836055) 3836055..3836333 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CA697_RS18535 (3836489) 3836489..3837229 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
CA697_RS18540 (3837200) 3837200..3837967 - 768 WP_000333380.1 class II glutamine amidotransferase -
CA697_RS18545 (3838173) 3838173..3838751 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T136766 WP_000615976.1 NZ_CP042969:3836055-3836333 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T136766 NZ_CP042969:3836055-3836333 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT136766 WP_000729704.1 NZ_CP042969:3835792-3836052 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT136766 NZ_CP042969:3835792-3836052 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References