Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1848308..1848810 Replicon chromosome
Accession NZ_CP042969
Organism Escherichia coli strain CFSAN061769

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CA697_RS08750 Protein ID WP_000767829.1
Coordinates 1848556..1848810 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CA697_RS08745 Protein ID WP_001259255.1
Coordinates 1848308..1848559 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA697_RS08720 (1843486) 1843486..1844553 - 1068 WP_000080067.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CA697_RS08725 (1844553) 1844553..1845623 - 1071 WP_085442794.1 histidinol-phosphate transaminase -
CA697_RS08730 (1845620) 1845620..1846924 - 1305 WP_023277243.1 histidinol dehydrogenase -
CA697_RS08735 (1846930) 1846930..1847829 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CA697_RS08740 (1847975) 1847975..1848025 - 51 WP_001364200.1 his operon leader peptide -
CA697_RS08745 (1848308) 1848308..1848559 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CA697_RS08750 (1848556) 1848556..1848810 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CA697_RS08755 (1848893) 1848893..1849717 + 825 WP_000754737.1 SDR family oxidoreductase -
CA697_RS08760 (1849763) 1849763..1850692 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
CA697_RS08765 (1850907) 1850907..1850969 + 63 WP_010723108.1 membrane protein YoeI -
CA697_RS08770 (1850959) 1850959..1852317 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CA697_RS08775 (1852496) 1852496..1853554 + 1059 WP_000492325.1 thiosulfate utilization transporter TsuA/YeeE -
CA697_RS08780 (1853568) 1853568..1853795 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T136752 WP_000767829.1 NZ_CP042969:1848556-1848810 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T136752 NZ_CP056222:1898685-1898787 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT136752 WP_001259255.1 NZ_CP042969:1848308-1848559 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT136752 NZ_CP056222:c1898794-1898649 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References