Detailed information of TA system TA136729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3739239..3739933
• Replicon: chromosome
• Accession: NZ_CP042946
• Organism: Escherichia fergusonii strain ATCC 35470

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: ABH49_RS18030
• Protein ID: WP_148047394.1
• Coordinates: 3739535..3739933 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ABH49_RS18025
• Protein ID: WP_000554757.1
• Coordinates: 3739239..3739532 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABH49_RS18010	3735592..3737331	-	1740	WP_105284586.1	flagellar biosynthesis protein FlhA	-
ABH49_RS18015	3737276..3738061	+	786	WP_172953827.1	putative lateral flagellar export/assembly protein LafU	-
ABH49_RS18020	3738132..3739187	+	1056	WP_001226157.1	DNA polymerase IV	-
ABH49_RS18025	3739239..3739532	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ABH49_RS18030	3739535..3739933	+	399	WP_148047394.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ABH49_RS18035	3739943..3740395	+	453	WP_148047395.1	GNAT family N-acetyltransferase	-
ABH49_RS18040	3740713..3741852	+	1140	WP_148047396.1	RNA ligase RtcB family protein	-
ABH49_RS18045	3741849..3742463	+	615	WP_148047397.1	peptide chain release factor H	-
ABH49_RS18050	3742520..3743977	-	1458	WP_148047398.1	cytosol nonspecific dipeptidase	-
ABH49_RS18055	3744238..3744696	+	459	WP_001291987.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15560.94 Da
• Isoelectric Point: 8.0934

>T136729 WP_148047394.1 NZ_CP042946:3739535-3739933 [Escherichia fergusonii]
MRVFKTKLIRLQLTDKELDALTADFISYKRDSVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T136729 NZ_CP042946:3739535-3739933 [Escherichia fergusonii]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGACAAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACAGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACA
AATGACGAAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT136729 WP_000554757.1 NZ_CP042946:3739239-3739532 [Escherichia fergusonii]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT136729 NZ_CP042946:3739239-3739532 [Escherichia fergusonii]
ATGCATCGAATTCTTGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATATTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGTTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6