Detailed information of TA system TA136724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2149927..2150726
• Replicon: chromosome
• Accession: NZ_CP042946
• Organism: Escherichia fergusonii strain ATCC 35470

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F0JQM9
• Locus tag: ABH49_RS10475
• Protein ID: WP_000347270.1
• Coordinates: 2150262..2150726 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: B7LMV4
• Locus tag: ABH49_RS10470
• Protein ID: WP_015953962.1
• Coordinates: 2149927..2150262 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABH49_RS10455	2146057..2146947	-	891	WP_000178100.1	2-hydroxy-3-oxopropionate reductase	-
ABH49_RS10460	2146972..2147742	-	771	WP_105284488.1	2-dehydro-3-deoxyglucarate aldolase	-
ABH49_RS10465	2148207..2149778	+	1572	WP_105284487.1	galactarate dehydratase	-
ABH49_RS10470	2149927..2150262	+	336	WP_015953962.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
ABH49_RS10475	2150262..2150726	+	465	WP_000347270.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
ABH49_RS10480	2150781..2151590	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
ABH49_RS10485	2151838..2153118	+	1281	WP_105284486.1	tagatose-bisphosphate aldolase subunit KbaZ	-
ABH49_RS10490	2153141..2153614	+	474	WP_105284485.1	PTS N-acetylgalactosamine transporter subunit IIB	-
ABH49_RS10495	2153625..2154404	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
ABH49_RS10500	2154394..2155272	+	879	WP_105284484.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
ABH49_RS10505	2155290..2155724	+	435	WP_000948819.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T136724 WP_000347270.1 NZ_CP042946:2150262-2150726 [Escherichia fergusonii]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T136724 NZ_CP056200:35700-36041 [Citrobacter sp. RHBSTW-00986]
ATGTGGGAAGTGGAAACAACGGATGTTTTCGATAAATGGTTTGACGTACAGACCGAGGCGCTCAAGGAAGATATGCTCGC
AGCGATGATGATTTTATCCGAGTATGGTCCTCAGTTAGGCAGGCCGTTTGCTGATACGGTAAATGCTTCTGCTTTTTCAA
ATATGAAAGAGTTGCGAGTGCAGCATCAGGGCAACCCGATTCGGGCGTTCTTCGTGTTTGATCCTTCTCGCCATGGCATC
GTGTTATGCGCAGGCGATAAAACAGGGTTAAACGAGAAGAAATTCTATAAAGAGATGATCAGGCTTGCTGACGCTGAGTA
CCGAAATCACCTTAATAAGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12281.89 Da
• Isoelectric Point: 4.4128

>AT136724 WP_015953962.1 NZ_CP042946:2149927-2150262 [Escherichia fergusonii]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDPTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT136724 NZ_CP042946:2149927-2150262 [Escherichia fergusonii]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGGCTGGACAGTATTCATTACGAAATTCTGCCTGGAGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCCTACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0V9NQ90

Antitoxin

• AlphaFold DB: B7LMV4