Detailed information of TA system TA136697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 867595..868274
• Replicon: chromosome
• Accession: NZ_CP042945
• Organism: Escherichia fergusonii strain ATCC 35471

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: FV159_RS04925
• Protein ID: WP_002431667.1
• Coordinates: 867972..868274 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FV159_RS04920
• Protein ID: WP_000806441.1
• Coordinates: 867595..867936 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FV159_RS04910	863381..864670	-	1290	WP_000102764.1	APC family permease	-
FV159_RS04915	864977..867481	+	2505	WP_000083966.1	copper-exporting P-type ATPase CopA	-
FV159_RS04920	867595..867936	-	342	WP_000806441.1	HigA family addiction module antidote protein	Antitoxin
FV159_RS04925	867972..868274	-	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FV159_RS04930	868407..869201	+	795	WP_000365145.1	TraB/GumN family protein	-
FV159_RS04935	869405..869884	+	480	WP_000186628.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
FV159_RS04940	869908..870708	+	801	WP_000439795.1	hypothetical protein	-
FV159_RS04945	870705..871208	+	504	WP_000667001.1	hypothetical protein	-
FV159_RS04950	871246..872898	-	1653	WP_000670595.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T136697 WP_002431667.1 NZ_CP042945:c868274-867972 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T136697 NZ_CP042945:c868274-867972 [Escherichia fergusonii]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCACTCAACGCCACATAGAAA
GATTCCTGCTGAAATCCATACCACATTATCAAGAAAATTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTATTTAGATCCACACAACTACTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13143.01 Da
• Isoelectric Point: 5.2345

>AT136697 WP_000806441.1 NZ_CP042945:c867936-867595 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKNVA

Nucleotide

• Length: 342 bp

>AT136697 NZ_CP056185:c5039346-5039104 [Citrobacter sp. RHBSTW-01013]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGAAGTGATCAAACGTCTTGATGATCTCAAGGT
GCAGCGTAATCTGCCGCGGGCTGAGCTTTTGCGGGAGGCTGTTGAACAGTATCTTGAGAGGCAGGATCAAGCTGAAACGA
CAATTTCACGTGCTCTTGGGCTATGGCAAGGTTGTGAGGAAGATGGCGTTGATTATCAGCGTAAACTCCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8