Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4177141..4177643 Replicon chromosome
Accession NZ_CP042942
Organism Escherichia fergusonii strain ATCC 35473

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FV158_RS20365 Protein ID WP_000767829.1
Coordinates 4177141..4177395 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FV158_RS20370 Protein ID WP_001259255.1
Coordinates 4177392..4177643 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FV158_RS20335 4172161..4172388 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
FV158_RS20340 4172402..4173460 - 1059 WP_046080827.1 transport protein YeeE -
FV158_RS20345 4173639..4174997 - 1359 WP_000019201.1 putrescine/proton symporter PlaP -
FV158_RS20350 4174987..4175049 - 63 WP_010723108.1 membrane protein YoeI -
FV158_RS20355 4175263..4176192 - 930 WP_148048545.1 LysR family transcriptional regulator -
FV158_RS20360 4176238..4177062 - 825 WP_000754745.1 SDR family oxidoreductase -
FV158_RS20365 4177141..4177395 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FV158_RS20370 4177392..4177643 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FV158_RS20375 4177927..4177977 + 51 WP_001364200.1 his operon leader peptide -
FV158_RS20380 4178123..4179022 + 900 WP_148048546.1 ATP phosphoribosyltransferase -
FV158_RS20385 4179028..4180332 + 1305 WP_148048547.1 histidinol dehydrogenase -
FV158_RS20390 4180329..4181399 + 1071 WP_148048548.1 histidinol-phosphate transaminase -
FV158_RS20395 4181399..4182466 + 1068 WP_148048549.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T136682 WP_000767829.1 NZ_CP042942:c4177395-4177141 [Escherichia fergusonii]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T136682 NZ_CP042942:c4177395-4177141 [Escherichia fergusonii]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATAGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT136682 WP_001259255.1 NZ_CP042942:c4177643-4177392 [Escherichia fergusonii]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT136682 NZ_CP042942:c4177643-4177392 [Escherichia fergusonii]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAACGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAAGAGACGGCTTATC
TATTGCGTTCCCCCGCTAATGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References