Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3550730..3551292 Replicon chromosome
Accession NZ_CP042942
Organism Escherichia fergusonii strain ATCC 35473

Toxin (Protein)


Gene name higB Uniprot ID E9XKD5
Locus tag FV158_RS17180 Protein ID WP_000605673.1
Coordinates 3551014..3551292 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag FV158_RS17175 Protein ID WP_000781370.1
Coordinates 3550730..3551014 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FV158_RS17160 3546089..3549136 + 3048 WP_148048369.1 formate dehydrogenase-N subunit alpha -
FV158_RS17165 3549149..3550033 + 885 WP_148048370.1 formate dehydrogenase N subunit beta -
FV158_RS17170 3550026..3550679 + 654 WP_000045645.1 formate dehydrogenase-N subunit gamma -
FV158_RS17175 3550730..3551014 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
FV158_RS17180 3551014..3551292 - 279 WP_000605673.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FV158_RS17185 3551478..3552488 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
FV158_RS17190 3552623..3554320 - 1698 WP_046082348.1 malate dehydrogenase -
FV158_RS17195 3554477..3554614 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
FV158_RS17200 3554716..3554931 - 216 WP_000495766.1 biofilm-dependent modulation protein -
FV158_RS17205 3555273..3555704 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10535.13 Da        Isoelectric Point: 7.3562

>T136681 WP_000605673.1 NZ_CP042942:c3551292-3551014 [Escherichia fergusonii]
MIMNFRHKGLRDLFLLGKTSGVIPTQIKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T136681 NZ_CP042942:c3551292-3551014 [Escherichia fergusonii]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTATTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAAT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGTGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTTGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT136681 WP_000781370.1 NZ_CP042942:c3551014-3550730 [Escherichia fergusonii]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT136681 NZ_CP042942:c3551014-3550730 [Escherichia fergusonii]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAGCTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCCGCGTTGACGCCAGAAATGGCGA
TTAAACTTTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTACAAAACGCCTGGAGTCTGGCAGAGGCAGAA
AAAACGGTAGATGTGTCGCGACTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7ADF6

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References