Detailed information of TA system TA136671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 2577471..2578150
• Replicon: chromosome
• Accession: NZ_CP042942
• Organism: Escherichia fergusonii strain ATCC 35473

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: FV158_RS12715
• Protein ID: WP_002431667.1
• Coordinates: 2577471..2577773 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FV158_RS12720
• Protein ID: WP_000806440.1
• Coordinates: 2577809..2578150 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FV158_RS12690	2572847..2574499	+	1653	WP_148048121.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
FV158_RS12695	2574537..2575040	-	504	WP_000667001.1	hypothetical protein	-
FV158_RS12700	2575037..2575837	-	801	WP_000439795.1	hypothetical protein	-
FV158_RS12705	2575861..2576340	-	480	WP_148048122.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
FV158_RS12710	2576544..2577338	-	795	WP_148048123.1	TraB/GumN family protein	-
FV158_RS12715	2577471..2577773	+	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FV158_RS12720	2577809..2578150	+	342	WP_000806440.1	HigA family addiction module antidote protein	Antitoxin
FV158_RS12725	2578264..2580768	-	2505	WP_148048124.1	copper-exporting P-type ATPase CopA	-
FV158_RS12730	2581075..2582364	+	1290	WP_148048125.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T136671 WP_002431667.1 NZ_CP042942:2577471-2577773 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T136671 NZ_CP042942:2577471-2577773 [Escherichia fergusonii]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCACTCAACGCCACATAGAAA
GATTCCTGCTGAAATCCATACCACATTATCAAGAAAATTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTATTTAGATCCACACAACTACTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13157.08 Da
• Isoelectric Point: 5.7737

>AT136671 WP_000806440.1 NZ_CP042942:2577809-2578150 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT136671 NZ_CP056169:84198-84464 [Enterobacter roggenkampii]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8