Detailed information of TA system TA136670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2402005..2402732
• Replicon: chromosome
• Accession: NZ_CP042942
• Organism: Escherichia fergusonii strain ATCC 35473

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0L6ZX69
• Locus tag: FV158_RS11795
• Protein ID: WP_000547560.1
• Coordinates: 2402005..2402316 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FV158_RS11800
• Protein ID: WP_000126291.1
• Coordinates: 2402313..2402732 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FV158_RS11765	2397146..2398855	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
FV158_RS11770	2398865..2399407	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
FV158_RS11775	2399407..2400174	+	768	WP_000067390.1	formate hydrogenlyase subunit HycG	-
FV158_RS11780	2400171..2400581	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
FV158_RS11785	2400574..2401044	+	471	WP_039021018.1	hydrogenase maturation peptidase HycI	-
FV158_RS11790	2401087..2401842	+	756	WP_039021019.1	hypothetical protein	-
FV158_RS11795	2402005..2402316	+	312	WP_000547560.1	type II toxin-antitoxin system HigB family toxin	Toxin
FV158_RS11800	2402313..2402732	+	420	WP_000126291.1	helix-turn-helix domain-containing protein	Antitoxin
FV158_RS11805	2402831..2403259	-	429	WP_148048061.1	DUF4259 domain-containing protein	-
FV158_RS11810	2403400..2404824	-	1425	WP_148048062.1	6-phospho-beta-glucosidase AscB	-
FV158_RS11815	2404833..2406290	-	1458	WP_148048063.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
FV158_RS11820	2406547..2407560	+	1014	WP_148048064.1	DNA-binding transcriptional regulator AscG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12423.12 Da
• Isoelectric Point: 9.7106

>T136670 WP_000547560.1 NZ_CP042942:2402005-2402316 [Escherichia fergusonii]
MHIISKAPFEESARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T136670 NZ_CP042942:2402005-2402316 [Escherichia fergusonii]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTCTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAATCTCGACAATTTTAAATATC
GCAATAAATGGTACGTTCTGGATGTAGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCATGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15439.46 Da
• Isoelectric Point: 4.5628

>AT136670 WP_000126291.1 NZ_CP042942:2402313-2402732 [Escherichia fergusonii]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNHEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT136670 NZ_CP042942:2402313-2402732 [Escherichia fergusonii]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGTATTGCAGAATATGAAAACAACCACGAAAAGTTTGCAGAGTTCGATAAAGCCGTTGCAGCAATGCCGGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAAAGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0L6ZX69