Detailed information of TA system TA136636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3286650..3287449
• Replicon: chromosome
• Accession: NZ_CP042934
• Organism: Escherichia coli 042

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: FVF73_RS16020
• Protein ID: WP_000347251.1
• Coordinates: 3286985..3287449 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: FVF73_RS16015
• Protein ID: WP_001307405.1
• Coordinates: 3286650..3286985 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVF73_RS16000	3282435..3283205	-	771	WP_001058214.1	2-dehydro-3-deoxyglucarate aldolase	-
FVF73_RS16005	3283221..3284555	-	1335	WP_000599652.1	galactarate/glucarate/glycerate transporter GarP	-
FVF73_RS16010	3284930..3286501	+	1572	WP_001273761.1	galactarate dehydratase	-
FVF73_RS16015	3286650..3286985	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
FVF73_RS16020	3286985..3287449	+	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
FVF73_RS16025	3287504..3288313	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
FVF73_RS16030	3288562..3289842	+	1281	WP_000681903.1	tagatose-bisphosphate aldolase subunit KbaZ	-
FVF73_RS16035	3289865..3290338	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
FVF73_RS16040	3290349..3291128	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
FVF73_RS16045	3291118..3291996	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
FVF73_RS16050	3292014..3292448	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3275775..3287449	11674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T136636 WP_000347251.1 NZ_CP042934:3286985-3287449 [Escherichia coli 042]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T136636 NZ_CP042934:3286985-3287449 [Escherichia coli 042]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT136636 WP_001307405.1 NZ_CP042934:3286650-3286985 [Escherichia coli 042]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT136636 NZ_CP042934:3286650-3286985 [Escherichia coli 042]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20

Antitoxin

• AlphaFold DB: A0A0E0XVC7