Detailed information of TA system TA136632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2831166..2831893
• Replicon: chromosome
• Accession: NZ_CP042934
• Organism: Escherichia coli 042

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: FVF73_RS13800
• Protein ID: WP_000547564.1
• Coordinates: 2831582..2831893 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FVF73_RS13795
• Protein ID: WP_000126294.1
• Coordinates: 2831166..2831585 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVF73_RS13775	2826216..2826743	-	528	WP_001078777.1	electron transport protein HydN	-
FVF73_RS13780	2826892..2827902	-	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
FVF73_RS13785	2828162..2829619	+	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
FVF73_RS13790	2829628..2831052	+	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
FVF73_RS13795	2831166..2831585	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
FVF73_RS13800	2831582..2831893	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
FVF73_RS13805	2832067..2832828	-	762	WP_001026446.1	hypothetical protein	-
FVF73_RS13810	2832853..2833323	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
FVF73_RS13815	2833316..2833726	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
FVF73_RS13820	2833723..2834490	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
FVF73_RS13825	2834490..2835032	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
FVF73_RS13830	2835042..2836751	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T136632 WP_000547564.1 NZ_CP042934:c2831893-2831582 [Escherichia coli 042]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T136632 NZ_CP042934:c2831893-2831582 [Escherichia coli 042]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT136632 WP_000126294.1 NZ_CP042934:c2831585-2831166 [Escherichia coli 042]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT136632 NZ_CP042934:c2831585-2831166 [Escherichia coli 042]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5