Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3961980..3962521 Replicon chromosome
Accession NZ_CP042903
Organism Escherichia coli strain CFSAN061761

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag CA899_RS19105 Protein ID WP_000615983.1
Coordinates 3962243..3962521 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag CA899_RS19100 Protein ID WP_000729703.1
Coordinates 3961980..3962240 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA899_RS19080 (3957756) 3957756..3958541 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
CA899_RS19085 (3958513) 3958513..3960225 + 1713 Protein_3757 flagellar biosynthesis protein FlhA -
CA899_RS19090 (3960348) 3960348..3960845 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
CA899_RS19095 (3961021) 3961021..3961770 - 750 WP_000056849.1 C40 family peptidase -
CA899_RS19100 (3961980) 3961980..3962240 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CA899_RS19105 (3962243) 3962243..3962521 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CA899_RS19110 (3962677) 3962677..3963417 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
CA899_RS19115 (3963388) 3963388..3964155 - 768 WP_000333380.1 class II glutamine amidotransferase -
CA899_RS19120 (3964361) 3964361..3964939 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T136585 WP_000615983.1 NZ_CP042903:3962243-3962521 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T136585 NZ_CP056148:c1058650-1058432 [Enterobacter roggenkampii]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACCCTCGAACGCGT
GATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCCGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAGC
TGACCATGAATAAGTTGTATGACAAAATCCCTACTTCTGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT136585 WP_000729703.1 NZ_CP042903:3961980-3962240 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT136585 NZ_CP042903:3961980-3962240 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References