Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3955884..3956578 Replicon chromosome
Accession NZ_CP042903
Organism Escherichia coli strain CFSAN061761

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag CA899_RS19065 Protein ID WP_001263489.1
Coordinates 3955884..3956282 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag CA899_RS19070 Protein ID WP_000554758.1
Coordinates 3956285..3956578 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3951472) 3951472..3951552 - 81 NuclAT_10 - -
- (3951472) 3951472..3951552 - 81 NuclAT_10 - -
- (3951472) 3951472..3951552 - 81 NuclAT_10 - -
- (3951472) 3951472..3951552 - 81 NuclAT_10 - -
CA899_RS19040 (3952148) 3952148..3952606 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
CA899_RS19045 (3952867) 3952867..3954324 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
CA899_RS19050 (3954381) 3954381..3954902 - 522 Protein_3750 peptide chain release factor H -
CA899_RS19055 (3954898) 3954898..3955104 - 207 Protein_3751 RtcB family protein -
CA899_RS19060 (3955422) 3955422..3955874 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
CA899_RS19065 (3955884) 3955884..3956282 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CA899_RS19070 (3956285) 3956285..3956578 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CA899_RS19075 (3956630) 3956630..3957685 - 1056 WP_001226164.1 DNA polymerase IV -
CA899_RS19080 (3957756) 3957756..3958541 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
CA899_RS19085 (3958513) 3958513..3960225 + 1713 Protein_3757 flagellar biosynthesis protein FlhA -
CA899_RS19090 (3960348) 3960348..3960845 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T136584 WP_001263489.1 NZ_CP042903:c3956282-3955884 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T136584 NZ_CP042903:c3956282-3955884 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT136584 WP_000554758.1 NZ_CP042903:c3956578-3956285 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT136584 NZ_CP042903:c3956578-3956285 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A090J8B1


Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References