136560

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3815085..3815626 Replicon chromosome
Accession NZ_CP042901
Organism Escherichia coli strain CFSAN061762

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag CA898_RS18550 Protein ID WP_000615983.1
Coordinates 3815348..3815626 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag CA898_RS18545 Protein ID WP_000729703.1
Coordinates 3815085..3815345 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA898_RS18525 (3810760) 3810760..3811545 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
CA898_RS18530 (3811517) 3811517..3813229 + 1713 Protein_3644 flagellar biosynthesis protein FlhA -
CA898_RS18535 (3813453) 3813453..3813950 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
CA898_RS18540 (3814126) 3814126..3814875 - 750 WP_024192679.1 C40 family peptidase -
CA898_RS18545 (3815085) 3815085..3815345 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CA898_RS18550 (3815348) 3815348..3815626 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CA898_RS18555 (3815782) 3815782..3816522 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
CA898_RS18560 (3816493) 3816493..3817260 - 768 WP_000333380.1 class II glutamine amidotransferase -
CA898_RS18565 (3817466) 3817466..3818044 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrB / gmhA/lpcA 3761140..3840990 79850


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T136560 WP_000615983.1 NZ_CP042901:3815348-3815626 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T136560 NZ_CP042901:3815348-3815626 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCATCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT136560 WP_000729703.1 NZ_CP042901:3815085-3815345 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT136560 NZ_CP042901:3815085-3815345 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
AlphaFold DB A0A5F1F716

References