Detailed information of TA system TA136538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4756232..4756834
• Replicon: chromosome
• Accession: NZ_CP042899
• Organism: Escherichia coli strain CFSAN061763

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CA897_RS22640
• Protein ID: WP_000897305.1
• Coordinates: 4756523..4756834 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CA897_RS22635
• Protein ID: WP_000356395.1
• Coordinates: 4756232..4756522 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA897_RS22600 (4751856)	4751856..4752758	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CA897_RS22605 (4752755)	4752755..4753390	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CA897_RS22610 (4753387)	4753387..4754316	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
CA897_RS22615 (4754498)	4754498..4754740	-	243	WP_001309881.1	CopG family transcriptional regulator	-
CA897_RS22620 (4754959)	4754959..4755177	-	219	WP_001295676.1	CopG family transcriptional regulator	-
CA897_RS22625 (4755596)	4755596..4755874	-	279	WP_001315112.1	hypothetical protein	-
CA897_RS22630 (4755926)	4755926..4756147	-	222	WP_001550354.1	hypothetical protein	-
CA897_RS22635 (4756232)	4756232..4756522	-	291	WP_000356395.1	NadS family protein	Antitoxin
CA897_RS22640 (4756523)	4756523..4756834	-	312	WP_000897305.1	hypothetical protein	Toxin
CA897_RS22645 (4757063)	4757063..4757971	+	909	WP_001550353.1	alpha/beta hydrolase	-
CA897_RS24410 (4758139)	4758139..4759053	-	915	WP_109553727.1	transposase	-
CA897_RS24415 (4759066)	4759066..4759953	-	888	Protein_4442	hypothetical protein	-
CA897_RS22655 (4760369)	4760369..4761310	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CA897_RS22660 (4761355)	4761355..4761792	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T136538 WP_000897305.1 NZ_CP042899:c4756834-4756523 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T136538 NZ_CP042899:c4756834-4756523 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT136538 WP_000356395.1 NZ_CP042899:c4756522-4756232 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT136538 NZ_CP042899:c4756522-4756232 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7