Detailed information of TA system TA136517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1113803..1114530
• Replicon: chromosome
• Accession: NZ_CP042899
• Organism: Escherichia coli strain CFSAN061763

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: CA897_RS05280
• Protein ID: WP_000547555.1
• Coordinates: 1113803..1114114 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CA897_RS05285
• Protein ID: WP_000126294.1
• Coordinates: 1114111..1114530 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA897_RS05255 (1109738)	1109738..1111447	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
CA897_RS05260 (1111457)	1111457..1111999	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
CA897_RS05265 (1111999)	1111999..1112766	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
CA897_RS05270 (1112763)	1112763..1113173	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
CA897_RS05275 (1113166)	1113166..1113636	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
CA897_RS05280 (1113803)	1113803..1114114	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
CA897_RS05285 (1114111)	1114111..1114530	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
CA897_RS05290 (1114609)	1114609..1116033	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
CA897_RS05295 (1116042)	1116042..1117499	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
CA897_RS05300 (1117759)	1117759..1118769	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
CA897_RS05305 (1118918)	1118918..1119445	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T136517 WP_000547555.1 NZ_CP042899:1113803-1114114 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T136517 NZ_CP042899:1113803-1114114 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT136517 WP_000126294.1 NZ_CP042899:1114111-1114530 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT136517 NZ_CP042899:1114111-1114530 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2