Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3783866..3784560 Replicon chromosome
Accession NZ_CP042896
Organism Escherichia coli strain CFSAN061771

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag CA902_RS17945 Protein ID WP_001263491.1
Coordinates 3783866..3784264 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag CA902_RS17950 Protein ID WP_000554755.1
Coordinates 3784267..3784560 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA902_RS17915 (3779128) 3779128..3780585 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
CA902_RS17920 (3780594) 3780594..3780875 + 282 WP_022645226.1 hypothetical protein -
CA902_RS17925 (3780892) 3780892..3781401 - 510 WP_001361775.1 metal-dependent hydrolase -
CA902_RS17930 (3781463) 3781463..3782077 - 615 WP_022645225.1 peptide chain release factor H -
CA902_RS17935 (3782074) 3782074..3783213 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
CA902_RS17940 (3783404) 3783404..3783856 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
CA902_RS17945 (3783866) 3783866..3784264 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CA902_RS17950 (3784267) 3784267..3784560 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CA902_RS17955 (3784612) 3784612..3785667 - 1056 WP_022645222.1 DNA polymerase IV -
CA902_RS17960 (3785738) 3785738..3786523 - 786 WP_000207551.1 putative lateral flagellar export/assembly protein LafU -
CA902_RS17965 (3786495) 3786495..3788207 + 1713 Protein_3534 flagellar biosynthesis protein FlhA -
CA902_RS17970 (3788305) 3788305..3789078 - 774 WP_022645219.1 C40 family peptidase -
CA902_RS17975 (3789264) 3789264..3789524 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T136501 WP_001263491.1 NZ_CP042896:c3784264-3783866 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T136501 NZ_CP056114:2958642-2958953 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT136501 WP_000554755.1 NZ_CP042896:c3784560-3784267 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT136501 NZ_CP042896:c3784560-3784267 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References