Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3642308..3642849 Replicon chromosome
Accession NZ_CP042893
Organism Escherichia coli strain CFSAN061772

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag CA901_RS17655 Protein ID WP_000615983.1
Coordinates 3642571..3642849 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag CA901_RS17650 Protein ID WP_000729703.1
Coordinates 3642308..3642568 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA901_RS17630 (3637990) 3637990..3638768 - 779 Protein_3456 putative lateral flagellar export/assembly protein LafU -
CA901_RS17635 (3638740) 3638740..3640452 + 1713 Protein_3457 flagellar biosynthesis protein FlhA -
CA901_RS17640 (3640676) 3640676..3641173 - 498 WP_116438975.1 REP-associated tyrosine transposase RayT -
CA901_RS17645 (3641349) 3641349..3642098 - 750 WP_000056849.1 C40 family peptidase -
CA901_RS17650 (3642308) 3642308..3642568 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CA901_RS17655 (3642571) 3642571..3642849 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CA901_RS17660 (3643005) 3643005..3643745 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
CA901_RS17665 (3643716) 3643716..3644483 - 768 WP_000333380.1 class II glutamine amidotransferase -
CA901_RS17670 (3644689) 3644689..3645267 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR / fha / tssJ / tssM / tssA / hcp1/tssD1 3625160..3665409 40249


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T136475 WP_000615983.1 NZ_CP042893:3642571-3642849 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T136475 NZ_CP042893:3642571-3642849 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT136475 WP_000729703.1 NZ_CP042893:3642308-3642568 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT136475 NZ_CP042893:3642308-3642568 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References