Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4699007..4699509 Replicon chromosome
Accession NZ_CP042892
Organism Escherichia coli O10:H32 strain NMBU-W12E19

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FVP48_RS24980 Protein ID WP_000767829.1
Coordinates 4699255..4699509 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FVP48_RS24975 Protein ID WP_001259255.1
Coordinates 4699007..4699258 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVP48_RS24950 4694185..4695252 - 1068 WP_000080091.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FVP48_RS24955 4695252..4696322 - 1071 WP_112022313.1 histidinol-phosphate transaminase -
FVP48_RS24960 4696319..4697623 - 1305 WP_032175486.1 histidinol dehydrogenase -
FVP48_RS24965 4697629..4698528 - 900 WP_000131756.1 ATP phosphoribosyltransferase -
FVP48_RS24970 4698674..4698724 - 51 WP_001364200.1 his operon leader peptide -
FVP48_RS24975 4699007..4699258 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FVP48_RS24980 4699255..4699509 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FVP48_RS24985 4699592..4700416 + 825 WP_032175485.1 SDR family oxidoreductase -
FVP48_RS24990 4700462..4701391 + 930 WP_000803363.1 LysR family transcriptional regulator -
FVP48_RS24995 4701606..4701668 + 63 WP_010723108.1 membrane protein YoeI -
FVP48_RS25000 4701658..4703016 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FVP48_RS25005 4703195..4704253 + 1059 WP_118020298.1 transport protein YeeE -
FVP48_RS25010 4704267..4704494 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T136448 WP_000767829.1 NZ_CP042892:4699255-4699509 [Escherichia coli O10:H32]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T136448 NZ_CP042892:4699255-4699509 [Escherichia coli O10:H32]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT136448 WP_001259255.1 NZ_CP042892:4699007-4699258 [Escherichia coli O10:H32]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT136448 NZ_CP042892:4699007-4699258 [Escherichia coli O10:H32]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCAAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References