Detailed information of TA system TA136439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1746368..1747062
• Replicon: chromosome
• Accession: NZ_CP042892
• Organism: Escherichia coli O10:H32 strain NMBU-W12E19

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: FVP48_RS11150
• Protein ID: WP_001263493.1
• Coordinates: 1746368..1746766 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FVP48_RS11155
• Protein ID: WP_000554757.1
• Coordinates: 1746769..1747062 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVP48_RS11120	1741368..1742612	-	1245	WP_000189541.1	esterase FrsA	-
-	1742028..1742108	-	81	NuclAT_10	-	-
-	1742028..1742108	-	81	NuclAT_10	-	-
-	1742028..1742108	-	81	NuclAT_10	-	-
-	1742028..1742108	-	81	NuclAT_10	-	-
FVP48_RS11125	1742704..1743162	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FVP48_RS11130	1743423..1744880	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
FVP48_RS11135	1744937..1745494	-	558	WP_001353768.1	peptide chain release factor H	-
FVP48_RS11140	1745406..1745672	-	267	WP_001321003.1	hypothetical protein	-
FVP48_RS11145	1745906..1746358	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
FVP48_RS11150	1746368..1746766	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FVP48_RS11155	1746769..1747062	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FVP48_RS11160	1747114..1748169	-	1056	WP_001226164.1	DNA polymerase IV	-
FVP48_RS11165	1748240..1749025	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
FVP48_RS11170	1748970..1750709	+	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
FVP48_RS11175	1750933..1751430	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T136439 WP_001263493.1 NZ_CP042892:c1746766-1746368 [Escherichia coli O10:H32]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T136439 NZ_CP042892:c1746766-1746368 [Escherichia coli O10:H32]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT136439 WP_000554757.1 NZ_CP042892:c1747062-1746769 [Escherichia coli O10:H32]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT136439 NZ_CP042892:c1747062-1746769 [Escherichia coli O10:H32]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6