Detailed information of TA system TA136429

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27456..27726
• Replicon: plasmid pNMBU-W12E19_03
• Accession: NZ_CP042887
• Organism: Escherichia coli O10:H32 strain NMBU-W12E19

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FVP48_RS01690
• Protein ID: WP_001312861.1
• Coordinates: 27568..27726 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	27456..27519 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVP48_RS01660	23167..23694	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
FVP48_RS01665	23752..23985	+	234	WP_000006003.1	DUF905 family protein	-
FVP48_RS01670	24046..26069	+	2024	Protein_35	ParB/RepB/Spo0J family partition protein	-
FVP48_RS01675	26138..26572	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FVP48_RS01680	26569..27288	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	27300..27524	+	225	NuclAT_0	-	-
-	27300..27524	+	225	NuclAT_0	-	-
-	27300..27524	+	225	NuclAT_0	-	-
-	27300..27524	+	225	NuclAT_0	-	-
FVP48_RS01685	27309..27488	-	180	WP_001309233.1	hypothetical protein	-
-	27456..27519	-	64	-	-	Antitoxin
FVP48_RS01690	27568..27726	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
FVP48_RS01695	28222..28415	+	194	Protein_40	hypothetical protein	-
FVP48_RS01700	28641..28937	+	297	WP_001272251.1	hypothetical protein	-
FVP48_RS01705	29048..29869	+	822	WP_001234445.1	DUF945 domain-containing protein	-
FVP48_RS01710	30166..30768	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
FVP48_RS01715	31091..31474	+	384	WP_001354030.1	relaxosome protein TraM	-
FVP48_RS01720	31668..32339	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
FVP48_RS01725	32476..32703	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A)	-	1..68601	68601

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T136429 WP_001312861.1 NZ_CP042887:27568-27726 [Escherichia coli O10:H32]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T136429 NZ_CP042887:27568-27726 [Escherichia coli O10:H32]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT136429 NZ_CP042887:c27519-27456 [Escherichia coli O10:H32]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin