Detailed information of TA system TA136393

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44794..45055
• Replicon: plasmid pNMBU-W05E18_02
• Accession: NZ_CP042880
• Organism: Escherichia coli strain NMBU_W05E18

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: -
• Locus tag: FVA71_RS26630
• Protein ID: WP_071608193.1
• Coordinates: 44794..44925 (-)
• Length: 44 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	44996..45055 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVA71_RS26595	39901..40434	-	534	WP_001221666.1	lipocalin family protein	-
FVA71_RS26600	40528..41673	-	1146	Protein_53	CMY-2 family class C beta-lactamase	-
FVA71_RS26605	42122..42815	-	694	Protein_54	IS1 family transposase	-
FVA71_RS26610	43039..43248	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
FVA71_RS26615	43640..43816	+	177	WP_001054897.1	hypothetical protein	-
FVA71_RS26620	43881..44177	-	297	WP_173009549.1	hypothetical protein	-
FVA71_RS26625	44472..44722	+	251	Protein_58	hypothetical protein	-
FVA71_RS26630	44794..44925	-	132	WP_071608193.1	Hok/Gef family protein	Toxin
-	44996..45055	+	60	NuclAT_1	-	Antitoxin
-	44996..45055	+	60	NuclAT_1	-	Antitoxin
-	44996..45055	+	60	NuclAT_1	-	Antitoxin
-	44996..45055	+	60	NuclAT_1	-	Antitoxin
FVA71_RS26635	45779..46925	+	1147	Protein_60	IS1380 family transposase	-
FVA71_RS26640	46980..47670	+	691	Protein_61	IS1 family transposase	-
FVA71_RS26645	47684..47989	-	306	Protein_62	transcription termination factor NusG	-
FVA71_RS26650	48431..48718	-	288	WP_000074855.1	conjugal transfer protein TraA	-
FVA71_RS26655	48636..48899	-	264	WP_032180564.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..49825	49825
	-	inside	IScluster/Tn	blaCMY-42	-	40744..46443	5699

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-39)

Sequences

Toxin

Protein

• Length: 44 a.a.
• Molecular weight: 4887.03 Da
• Isoelectric Point: 8.2781

>T136393 WP_071608193.1 NZ_CP042880:c44925-44794 [Escherichia coli]
MMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 132 bp

>T136393 NZ_CP042880:c44925-44794 [Escherichia coli]
ATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTCGCTTTGCGGACTCCGGCTCCA
GCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT136393 NZ_CP042880:44996-45055 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAATCTCCGTGGGCTTTCTGCTGT

Structures

Antitoxin