Detailed information of TA system TA136389

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 114015..114441
• Replicon: plasmid pNMBU-W05E18_01
• Accession: NZ_CP042879
• Organism: Escherichia coli strain NMBU_W05E18

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FVA71_RS26190
• Protein ID: WP_001312861.1
• Coordinates: 114015..114173 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	114217..114441 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVA71_RS26160	109412..110101	-	690	WP_000283387.1	conjugal transfer transcriptional regulator TraJ	-
FVA71_RS26165	110288..110671	-	384	WP_001151538.1	relaxosome protein TraM	-
FVA71_RS26170	110992..111594	+	603	Protein_133	transglycosylase SLT domain-containing protein	-
FVA71_RS26175	111891..112712	-	822	WP_001234445.1	DUF945 domain-containing protein	-
FVA71_RS26180	112822..113118	-	297	WP_001545326.1	hypothetical protein	-
FVA71_RS26185	113418..113714	+	297	Protein_136	hypothetical protein	-
FVA71_RS26190	114015..114173	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	114217..114441	-	225	NuclAT_0	-	Antitoxin
-	114217..114441	-	225	NuclAT_0	-	Antitoxin
-	114217..114441	-	225	NuclAT_0	-	Antitoxin
-	114217..114441	-	225	NuclAT_0	-	Antitoxin
FVA71_RS26195	114253..114432	+	180	WP_001309233.1	hypothetical protein	-
FVA71_RS26200	114453..115172	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
FVA71_RS26205	115169..115603	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FVA71_RS26210	115672..117636	-	1965	WP_001537566.1	ParB/RepB/Spo0J family partition protein	-
FVA71_RS26215	117697..117930	-	234	WP_000006003.1	DUF905 family protein	-
FVA71_RS26220	117988..118515	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
FVA71_RS26225	118817..119254	+	438	WP_024185955.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..132874	132874

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T136389 WP_001312861.1 NZ_CP042879:c114173-114015 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T136389 NZ_CP042879:c114173-114015 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT136389 NZ_CP042879:c114441-114217 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin