Detailed information of TA system TA136385

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66894..67147
• Replicon: plasmid pNMBU-W05E18_01
• Accession: NZ_CP042879
• Organism: Escherichia coli strain NMBU_W05E18

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: FVA71_RS25910
• Protein ID: WP_001336447.1
• Coordinates: 66894..67043 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	67091..67147 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVA71_RS25870	62369..63139	+	771	WP_000712166.1	molybdopterin-dependent oxidoreductase	-
FVA71_RS25875	63169..63417	+	249	WP_000738203.1	hypothetical protein	-
FVA71_RS25880	63567..63665	-	99	WP_137491101.1	transposase	-
FVA71_RS25885	63858..64166	+	309	Protein_76	transposase	-
FVA71_RS25890	64281..64493	-	213	WP_001336932.1	hypothetical protein	-
FVA71_RS25895	65194..66051	-	858	WP_001537577.1	incFII family plasmid replication initiator RepA	-
FVA71_RS25900	66044..66118	-	75	WP_001365705.1	RepA leader peptide Tap	-
FVA71_RS25905	66352..66609	-	258	WP_000084404.1	replication regulatory protein RepA	-
FVA71_RS25910	66894..67043	-	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	67091..67147	+	57	NuclAT_1	-	Antitoxin
-	67091..67147	+	57	NuclAT_1	-	Antitoxin
-	67091..67147	+	57	NuclAT_1	-	Antitoxin
-	67091..67147	+	57	NuclAT_1	-	Antitoxin
FVA71_RS25915	67242..67679	-	438	WP_000872609.1	hypothetical protein	-
FVA71_RS25920	67832..68215	-	384	WP_001109264.1	hypothetical protein	-
FVA71_RS25925	68318..68779	-	462	WP_000760080.1	thermonuclease family protein	-
FVA71_RS25930	69887..70444	-	558	WP_000139329.1	fertility inhibition protein FinO	-
FVA71_RS25935	70499..71245	-	747	WP_000205709.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	63900..64166	266
	-	inside	Conjugative plasmid	-	senB	1..132874	132874

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T136385 WP_001336447.1 NZ_CP042879:c67043-66894 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T136385 NZ_CP042879:c67043-66894 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT136385 NZ_CP042879:67091-67147 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin