Detailed information of TA system TA136384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5096809..5097411
• Replicon: chromosome
• Accession: NZ_CP042878
• Organism: Escherichia coli strain NMBU_W05E18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FVA71_RS24565
• Protein ID: WP_000897305.1
• Coordinates: 5097100..5097411 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FVA71_RS24560
• Protein ID: WP_000356395.1
• Coordinates: 5096809..5097099 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVA71_RS24525	5092433..5093335	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FVA71_RS24530	5093332..5093967	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FVA71_RS24535	5093964..5094893	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
FVA71_RS24540	5095075..5095317	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
FVA71_RS24545	5095536..5095754	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
FVA71_RS24550	5096173..5096451	-	279	WP_001315112.1	hypothetical protein	-
FVA71_RS24555	5096503..5096724	-	222	WP_001550354.1	hypothetical protein	-
FVA71_RS24560	5096809..5097099	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
FVA71_RS24565	5097100..5097411	-	312	WP_000897305.1	hypothetical protein	Toxin
FVA71_RS24570	5097640..5098548	+	909	WP_001550353.1	alpha/beta hydrolase	-
FVA71_RS24575	5098716..5100530	-	1815	WP_001550352.1	hypothetical protein	-
FVA71_RS24580	5100946..5101887	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FVA71_RS24585	5101932..5102369	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T136384 WP_000897305.1 NZ_CP042878:c5097411-5097100 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T136384 NZ_CP042878:c5097411-5097100 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT136384 WP_000356395.1 NZ_CP042878:c5097099-5096809 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT136384 NZ_CP055883:c3851385-3851119 [Enterobacter sp. RHBSTW-00975]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGATGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCTGATT
TATTTAACTGGCTGATGAATCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7