Detailed information of TA system TA136359

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2550381..2550752
• Replicon: chromosome
• Accession: NZ_CP042878
• Organism: Escherichia coli strain NMBU_W05E18

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: FVA71_RS12305
• Protein ID: WP_001317028.1
• Coordinates: 2550558..2550752 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2550381..2550559 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVA71_RS12280	2546336..2547709	+	1374	WP_001551034.1	ATP-dependent RNA helicase DbpA	-
FVA71_RS12285	2547838..2548773	-	936	WP_000662472.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
FVA71_RS12290	2548825..2550060	-	1236	WP_001551035.1	site-specific integrase	-
FVA71_RS12295	2550062..2550277	-	216	WP_000079604.1	excisionase XisR	-
FVA71_RS12300	2550377..2550565	-	189	WP_001551036.1	DUF1187 family protein	-
-	2550381..2550559	+	179	NuclAT_0	-	Antitoxin
-	2550381..2550559	+	179	NuclAT_0	-	Antitoxin
-	2550381..2550559	+	179	NuclAT_0	-	Antitoxin
-	2550381..2550559	+	179	NuclAT_0	-	Antitoxin
FVA71_RS12305	2550558..2550752	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
FVA71_RS12310	2550809..2551618	-	810	WP_001551037.1	recombination protein RecT	-
FVA71_RS12315	2551611..2554262	-	2652	WP_001551038.1	exodeoxyribonuclease VIII	-
FVA71_RS12320	2554364..2554639	-	276	WP_001314664.1	hypothetical protein	-
FVA71_RS12325	2554714..2554884	-	171	WP_001551041.1	phage protein	-
FVA71_RS12330	2554884..2555105	-	222	WP_000560221.1	killing protein KilR	-
FVA71_RS12335	2555526..2555678	-	153	WP_001551042.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2548825..2596116	47291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T136359 WP_001317028.1 NZ_CP042878:c2550752-2550558 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T136359 NZ_CP042878:c2550752-2550558 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT136359 NZ_CP042878:2550381-2550559 [Escherichia coli]
GAGGACTGAAGTCTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGGCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin