Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 713192..713991 Replicon chromosome
Accession NZ_CP042878
Organism Escherichia coli strain NMBU_W05E18

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag FVA71_RS03420 Protein ID WP_000347270.1
Coordinates 713192..713656 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag FVA71_RS03425 Protein ID WP_001551693.1
Coordinates 713656..713991 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVA71_RS03390 708193..708627 - 435 WP_000948837.1 PTS sugar transporter subunit IIA -
FVA71_RS03395 708645..709523 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
FVA71_RS03400 709513..710292 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
FVA71_RS03405 710303..710776 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
FVA71_RS03410 710799..712079 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
FVA71_RS03415 712328..713137 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
FVA71_RS03420 713192..713656 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
FVA71_RS03425 713656..713991 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
FVA71_RS03430 714140..715711 - 1572 WP_001273738.1 galactarate dehydratase -
FVA71_RS03435 716086..717420 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
FVA71_RS03440 717436..718206 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T136353 WP_000347270.1 NZ_CP042878:c713656-713192 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T136353 NZ_CP055599:3843210-3843362 [Escherichia fergusonii]
ATGCTGACAAAATATGCCCTTGTGGCAATCATCGTACTGTGTTGTACAGTACTGGGATTCACGCTGATGGTAGGCGACTC
GTTGTGTGAGTTGAGTATCAGAGAACGTGGTATGGAGTTTAAGGCAGTTCTCGCTTACGAATCGAAGAAGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT136353 WP_001551693.1 NZ_CP042878:c713991-713656 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT136353 NZ_CP042878:c713991-713656 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References