Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3676064..3676605 Replicon chromosome
Accession NZ_CP042867
Organism Escherichia coli strain ATCC BAA-196

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag BAA196FS_RS18080 Protein ID WP_000615983.1
Coordinates 3676327..3676605 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag BAA196FS_RS18075 Protein ID WP_000729703.1
Coordinates 3676064..3676324 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAA196FS_RS18055 3671739..3672524 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
BAA196FS_RS18060 3672469..3674208 + 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
BAA196FS_RS18065 3674432..3674929 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
BAA196FS_RS18070 3675105..3675854 - 750 WP_000056849.1 C40 family peptidase -
BAA196FS_RS18075 3676064..3676324 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
BAA196FS_RS18080 3676327..3676605 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
BAA196FS_RS18085 3676761..3677501 + 741 WP_001225679.1 murein L,D-transpeptidase -
BAA196FS_RS18090 3677472..3678239 - 768 WP_000333380.1 class II glutamine amidotransferase -
BAA196FS_RS18095 3678445..3679023 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T136343 WP_000615983.1 NZ_CP042867:3676327-3676605 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T136343 NZ_CP055599:1532661-1532879 [Escherichia fergusonii]
ATGTCCGATAAGCCTTTAACCAAAACTGATTATTTGATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAACGTGT
CATCGAAAAAAATAAATACGAACTCTCTGACAATGAACTGGCGGTATTTTATTCAGCAGCTGATCACCGTCTCGCTGAAT
TGACCATGAATAAACTTTACGACAAGATCCCCTCCTCCGTATGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT136343 WP_000729703.1 NZ_CP042867:3676064-3676324 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT136343 NZ_CP055599:1532260-1532634 [Escherichia fergusonii]
ATGGACGAATACTCACCAAAACGACATGATATCGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAATCTTGAAGAGAGCAACCATGGCTGGGTTAACGACCCAACCTCGGCTATCAATCTGCAGCTCAACGAACTGATTGAGC
ATATTGCAACCTTCGCACTTAATTACAAAATTAAGTATAATGAGGACAATAAGCTCATAGAGCAGATCGATGAATATCTG
GATGACACTTTTATGTTGTTCAGTAGTTATGGCATTAATACACAGGATTTACAGAAATGGCGAAAATCTGGAAACAGGCT
CTTCCGCTGTTTTGTTAACGCCACCAGGGCGAACCCTGTTAGTTTGTCGTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References