Detailed information of TA system TA136342

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3669867..3670561
• Replicon: chromosome
• Accession: NZ_CP042867
• Organism: Escherichia coli strain ATCC BAA-196

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: BAA196FS_RS18040
• Protein ID: WP_001263489.1
• Coordinates: 3669867..3670265 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: BAA196FS_RS18045
• Protein ID: WP_000554758.1
• Coordinates: 3670268..3670561 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3665455..3665535	-	81	NuclAT_12	-	-
-	3665455..3665535	-	81	NuclAT_12	-	-
-	3665455..3665535	-	81	NuclAT_12	-	-
-	3665455..3665535	-	81	NuclAT_12	-	-
BAA196FS_RS18015	3666131..3666589	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
BAA196FS_RS18020	3666850..3668307	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
BAA196FS_RS18025	3668364..3668921	-	558	WP_001352051.1	peptide chain release factor H	-
BAA196FS_RS18030	3668833..3669099	-	267	WP_001295202.1	hypothetical protein	-
BAA196FS_RS18035	3669405..3669857	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
BAA196FS_RS18040	3669867..3670265	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BAA196FS_RS18045	3670268..3670561	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BAA196FS_RS18050	3670613..3671668	-	1056	WP_001226164.1	DNA polymerase IV	-
BAA196FS_RS18055	3671739..3672524	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
BAA196FS_RS18060	3672469..3674208	+	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
BAA196FS_RS18065	3674432..3674929	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T136342 WP_001263489.1 NZ_CP042867:c3670265-3669867 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T136342 NZ_CP042867:c3670265-3669867 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT136342 WP_000554758.1 NZ_CP042867:c3670561-3670268 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT136342 NZ_CP042867:c3670561-3670268 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3