Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1798038..1798540 Replicon chromosome
Accession NZ_CP042865
Organism Escherichia coli strain ATCC BAA-196

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BAA196NC_RS08545 Protein ID WP_000767829.1
Coordinates 1798286..1798540 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BAA196NC_RS08540 Protein ID WP_001259255.1
Coordinates 1798038..1798289 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAA196NC_RS08515 1793216..1794283 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
BAA196NC_RS08520 1794283..1795353 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
BAA196NC_RS08525 1795350..1796654 - 1305 WP_000009594.1 histidinol dehydrogenase -
BAA196NC_RS08530 1796660..1797559 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
BAA196NC_RS08535 1797705..1797755 - 51 WP_001364200.1 his operon leader peptide -
BAA196NC_RS08540 1798038..1798289 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BAA196NC_RS08545 1798286..1798540 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BAA196NC_RS08550 1798623..1799447 + 825 WP_000754737.1 SDR family oxidoreductase -
BAA196NC_RS08555 1799493..1800422 + 930 WP_000803351.1 LysR family transcriptional regulator -
BAA196NC_RS08560 1800637..1800699 + 63 WP_010723108.1 membrane protein YoeI -
BAA196NC_RS08565 1800689..1802047 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BAA196NC_RS08570 1802226..1803284 + 1059 WP_000492339.1 transport protein YeeE -
BAA196NC_RS08575 1803298..1803525 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T136297 WP_000767829.1 NZ_CP042865:1798286-1798540 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T136297 NZ_CP055418:3978217-3978558 [Citrobacter sp. RHBSTW-00944]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGCGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT136297 WP_001259255.1 NZ_CP042865:1798038-1798289 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT136297 NZ_CP042865:1798038-1798289 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References