Detailed information of TA system TA136264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4886965..4887622
• Replicon: chromosome
• Accession: NZ_CP042858
• Organism: Klebsiella pneumoniae strain QD23

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: FU841_RS24905
• Protein ID: WP_000270043.1
• Coordinates: 4886965..4887315 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FU841_RS24910
• Protein ID: WP_000124640.1
• Coordinates: 4887320..4887622 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FU841_RS24860	4882520..4883224	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FU841_RS24865	4883439..4883936	-	498	WP_000062185.1	hypothetical protein	-
FU841_RS24870	4883939..4884427	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
FU841_RS24875	4884524..4884859	+	336	WP_000683476.1	hypothetical protein	-
FU841_RS24880	4884874..4885344	-	471	WP_001281821.1	hypothetical protein	-
FU841_RS24885	4885337..4885708	-	372	WP_000516916.1	hypothetical protein	-
FU841_RS24890	4885719..4885913	-	195	WP_000343597.1	hypothetical protein	-
FU841_RS24900	4886254..4886802	-	549	WP_001061574.1	transcriptional regulator	-
FU841_RS24905	4886965..4887315	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FU841_RS24910	4887320..4887622	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
FU841_RS24915	4887649..4887942	-	294	WP_001239997.1	chromosome segregation protein ParM	-
FU841_RS24920	4888030..4888302	-	273	WP_001043047.1	HU family DNA-binding protein	-
FU841_RS24925	4888360..4888887	-	528	WP_001236377.1	thermonuclease family protein	-
FU841_RS24935	4889118..4889975	-	858	WP_001167032.1	hypothetical protein	-
FU841_RS24940	4889962..4890192	-	231	WP_000972663.1	hypothetical protein	-
FU841_RS24945	4890192..4890710	-	519	WP_000210756.1	nitrite reductase	-
FU841_RS24950	4890707..4891153	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
FU841_RS24955	4891153..4891512	-	360	WP_000422768.1	hypothetical protein	-
FU841_RS24960	4891569..4891997	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aadA2 / ere(A) / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / aac(3)-IVa / aph(4)-Ia / sul2 / floR / blaCTX-M-65 / fosA3 / aph(3')-Ia / tet(C) / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / mph(A) / dfrA12 / blaTEM-1B / blaNDM-3 / aadA16 / dfrA27	-	4373245..4915543	542298

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T136264 WP_000270043.1 NZ_CP042858:4886965-4887315 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T136264 NZ_CP042858:4886965-4887315 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT136264 WP_000124640.1 NZ_CP042858:4887320-4887622 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT136264 NZ_CP042858:4887320-4887622 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9