Detailed information of TA system TA136261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4740628..4741355
• Replicon: chromosome
• Accession: NZ_CP042858
• Organism: Klebsiella pneumoniae strain QD23

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: FU841_RS24025
• Protein ID: WP_011251285.1
• Coordinates: 4741044..4741355 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FU841_RS24020
• Protein ID: WP_011251286.1
• Coordinates: 4740628..4741047 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FU841_RS23995	4736220..4737587	+	1368	WP_017900870.1	formimidoylglutamate deiminase	-
FU841_RS24000	4738112..4738647	+	536	Protein_4620	transposase	-
FU841_RS24005	4738761..4739027	-	267	WP_017900868.1	hypothetical protein	-
FU841_RS24010	4739131..4740099	+	969	Protein_4622	IS5 family transposase	-
FU841_RS24015	4740145..4740558	-	414	WP_017901337.1	hypothetical protein	-
FU841_RS24020	4740628..4741047	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
FU841_RS24025	4741044..4741355	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
FU841_RS24030	4741560..4742018	-	459	Protein_4626	DDE-type integrase/transposase/recombinase	-
FU841_RS24035	4742111..4742488	+	378	WP_004118218.1	IS66 family insertion sequence hypothetical protein	-
FU841_RS24040	4742485..4742832	+	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
FU841_RS24045	4742881..4744419	+	1539	WP_017901237.1	IS66 family transposase	-
FU841_RS24050	4744459..4744638	+	180	Protein_4630	IS5/IS1182 family transposase	-
FU841_RS24055	4745184..4746164	-	981	WP_012569499.1	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aadA2 / ere(A) / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / aac(3)-IVa / aph(4)-Ia / sul2 / floR / blaCTX-M-65 / fosA3 / aph(3')-Ia / tet(C) / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / mph(A) / dfrA12 / blaTEM-1B / blaNDM-3 / aadA16 / dfrA27	-	4373245..4915543	542298
	-	inside	IScluster/Tn	-	-	4738519..4746164	7645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T136261 WP_011251285.1 NZ_CP042858:c4741355-4741044 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T136261 NZ_CP042858:c4741355-4741044 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT136261 WP_011251286.1 NZ_CP042858:c4741047-4740628 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT136261 NZ_CP055302:c188828-188595 [Mannheimia pernigra]
ATGATTGAAGCTTCAATTTTTATGACTAATCGAAGTCAAGCGGTTCGCTTGCCTGCTGAAGTTCGTTTTGATAATGACGT
AAAAAAAATAGCAGTTCGCATTTTAGGAAATGAACGTATTTTGTCTCCTCTCAACCAAAGTTGGGATAGTTTTTTTCTTA
ATAGTGAAACCGTCAGTGAGGACTTTATGGTCGAAAGAGAAATAGCAATGCAGCCTGAGCGAGAGGATTTCTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1