Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2787930..2788432 Replicon chromosome
Accession NZ_CP042846
Organism Escherichia coli strain JME64

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FVE20_RS13750 Protein ID WP_000767829.1
Coordinates 2787930..2788184 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FVE20_RS13755 Protein ID WP_001259255.1
Coordinates 2788181..2788432 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE20_RS13720 2782945..2783172 - 228 WP_000234896.1 sulfurtransferase TusA -
FVE20_RS13725 2783186..2784244 - 1059 WP_000492339.1 transport protein YeeE -
FVE20_RS13730 2784423..2785781 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FVE20_RS13735 2785771..2785833 - 63 WP_010723108.1 membrane protein YoeI -
FVE20_RS13740 2786048..2786977 - 930 WP_000803351.1 LysR family transcriptional regulator -
FVE20_RS13745 2787023..2787847 - 825 WP_000754737.1 SDR family oxidoreductase -
FVE20_RS13750 2787930..2788184 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FVE20_RS13755 2788181..2788432 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FVE20_RS13760 2788715..2788765 + 51 WP_001364200.1 his operon leader peptide -
FVE20_RS13765 2788911..2789810 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
FVE20_RS13770 2789816..2791120 + 1305 WP_000009594.1 histidinol dehydrogenase -
FVE20_RS13775 2791117..2792187 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
FVE20_RS13780 2792187..2793254 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T136230 WP_000767829.1 NZ_CP042846:c2788184-2787930 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T136230 NZ_CP042846:c2788184-2787930 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT136230 WP_001259255.1 NZ_CP042846:c2788432-2788181 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT136230 NZ_CP042846:c2788432-2788181 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References